α2-Heremans-Schimid糖蛋白基因多态性与脑梗死相关性的探讨  

Association betweenα2-Heremans-Schimid glycoprotein （AHSG） polymorphism and cerebral infarction

作　　者：吴杨[1] 侯宏伟 杨丛林 陆洋 辛显峰 于洋[1] 刘家丰[1] 

机构地区：[1]哈尔滨市第一医院神经内四科,黑龙江哈尔滨150010 [2]哈尔滨市第五医院神经内科,黑龙江哈尔滨150040 [3]哈尔滨市第五医院骨科,黑龙江哈尔滨150040

出　　处：《中国急救医学》2014年第11期1017-1021,共5页Chinese Journal of Critical Care Medicine

基　　金：黑龙江省自然科学基金（D201176）

摘　　要：目的探讨α2-Heremans-Schimid-糖蛋白（AHSG）的5个单核苷酸多态性位点（rs4917、rs4918、rs1071592、rs2070633、rs2070635）与脑梗死的相关性。方法采用直接测序法对200例脑梗死患者及100例健康对照者进行5个多态性位点检测，检测结果以Hardy-Weinberg平衡检验观察样本是否具有群体代表性。χ2检验对计量资料、基因型和等位基因频率分布进行检验。基因型和等位基因风险率采用相对危险度（OR）表示。结果AHSG基因的rs4917位点的CC、CT、TT基因型在脑梗死组和对照组间比较差异无统计学意义（χ2＝5．243，P＝0．079）。CC基因型频率在脑梗死组显著高于对照组（χ2＝5．230，P＝0．027），CC基因型患脑梗死风险较CT＋TT基因型高（OR＝1．758，95％CI1．082-2．856）。C等位基因频率在两组间比较差异有统计学意义（χ2＝4．758，P＝0．032，OR＝1．492，95％CI1．040-2．139）。rs4918位点的CC、CG、GG基因型在脑梗死组和对照组间比较差异有统计学意义（χ2＝7．335，P＝0．024）。GG基因型频率在脑梗死组显著高于对照组（χ2＝5．055，P＝0．028），GG基因型患脑梗死风险较CG＋CC基因型高（OR＝2．965，95％CI1．106-7．953）。G等位基因在脑梗死组中的频率高于对照组，但差异无统计学意义（χ2＝0．408，P＝0．566）。rs1071592位点的CC、AC、AA基因型在脑梗死组和对照组间比较差异无统计学意义（χ2＝5．375，P＝0．065）。AA基因型频率在脑梗死组显著高于对照组（χ2＝4．615，P＝0．037），AA基因型患脑梗死风险较AC＋CC基因型高（OR＝3．593，95％CI1．041-12．394）。A等位基因在脑梗死组中的频率高于对照组，但差异无统计学意义（χ2＝0.942，P＝0．351，OR＝1．229，95％CI0．810-1．864）。两组rs2070633、rs2070635位点基因型及等位基因频率分布及相对风险分析比较差异无统计学意义（P＞0．05）。结�Objective To investigate the relationship between five single -nucleotide polymorphism （SNP） ofα2-Heremans-Schimid glycoprotein （AHSG） and cerebral infarction（CI）. Methods Five gene polymorphisms were detected for 200 patients of CI and 100 healthy controls using direct sequencing method.The χ2 test was adopted to check the difference in genotype and allele frequency distribution between the cases and the controls, the accordance with Hardy-Weinberg equilibrium in the group, and genotype and allele risk rate were showed with odds ratio （ OR） .Results There were no significant differences（χ2 =5.243,P=0.079） between CI and healthy control in CC, CT, TT genotypes of rs4917 of AHSG gene.The frequency of CC genotype in CI group was significantly higher than in the control group（χ2 =5.230,P=0.027）.And the diseased risk of CI in genotype CC＆amp;nbsp;was higher than CT＋TT （OR=1.758,95%CI 1.082-2.856）.The frequency of C allele between CI and healthy control had significant differences （χ2 =4.758,P=0.032,OR=1.492,95%CI 1.040-2.139） .The frequencies of CC, CG, GG of rs4018 of AHSG gene had significant difference between two groups（χ2 =7.335,P=0.024）.Compared with genotype CG＋CC, the frequency of genotype GG in CI was higher than in the control group（χ2 =5.055,P=0.028） .And the diseased risk of CI in genotype GG was higher than CG＋CC （OR=2.965,95%CI 1.106 -7.953）.The frequency of G allele in CI was higher than in the control group, which had no significant differences（χ2 =0.408,P=0.566） .The frequencies of CC, AC, AA of 1071592 of AHSG gene had no significant difference between two groups（χ2 =5.375,P=0.065）.Compared with genotype AC ＋CC, the frequency of genotype AA in CI was significantly higher than in the control group（χ2 =4.615,P=0.037）, and the diseased risk of CI in genotype AA was higher than AC＋CC （OR=3.593,95%CI 1.041-12.394）. The frequency of A allele in CI was higher than in the control group, but which had no significant differences

关 键 词：α2-Heremans-Schimid糖蛋白(AHSG) 基因多态性 脑梗死 

分 类 号：R743[医药卫生—神经病学与精神病学]