甘草酸二铵对百草枯中毒大鼠肺组织TLR-4mRNA表达的影响  

Diammonium glycyrrhizinate down-regulating lung Toll-like receptor 4 mRNA level in acute lung injury rats induced by paraquat

作　　者：林莉[1] 李浩[1] 张剑锋[1] 张伟[1] 苏思标[1] 

机构地区：[1]广西医科大学第一附属医院急诊科,广西南宁530007

出　　处：《中国急救医学》2014年第11期1029-1032,共4页Chinese Journal of Critical Care Medicine

基　　金：国家自然科学基金项目（81360290）;广西自然科学基金资助项目（2014GXNXFAA118195）

摘　　要：目的探讨甘草酸二铵（DG）对百草枯中毒（PQ）致急性肺损伤（ALI）大鼠肺Toll样受体4（TLR-4）mRNA、NF-κBmRNA表达及对血清白细胞介素-6（IL-6）、白细胞介素-17（IL-17）浓度的影响。方法将50只SD大鼠采用随机数字表法分为PQ组（n＝20）、DG治疗组（n＝20）和正常对照组（S组，n＝10），PQ组和DG治疗组予百草枯100mg/kg灌胃1次，DG组于灌胃后立即腹腔注射DG50mg/kg（1次/d），S组和PQ组注射等剂量的生理盐水，观察至48h。取肺组织检测肺湿干质量比（W/D）；采用RT-PCR法检测肺组织TLR-4mRNA、NF-κBmRNA的表达情况；ELISA法测定血清IL-6、IL-17浓度。另选择健康SD大鼠50只，分组及各组处置方法同上，观察其7d内死亡率。结果与S组比较，PQ组W/D、TLR-4mRNA、NF-κBmRNA表达均明显升高（P＜0．01），血清IL-6、IL-17亦升高（P＜0．01）；DG组干预后，W/D降低，TLR-4mRNA、NF-kBmRN、AIL-6及IL-17有所降低，与PQ组比较差异有统计学意义（P＜0.01或P＜0．05）。PQ组大鼠7d死亡18只（死亡率90％），DG组死亡8只（死亡率40％），NS组无死亡（死亡率0）。结论甘草酸二铵可抑制PQ致ALI大鼠肺组织TLR-4mRNA、NF-κBmRNA及血清IL-6、IL-17浓度的过度表达，从而减轻肺损伤程度。Objective To evaluate the effect of diammonium glycyrrhizinate （ DG ） on paraquat-induced acute lung injury in rats.Methods Fifty SD rats were randomly divided into control group （ NS ） , PQ group and DG group.The rats in PQ group and DG group were intragastriced administration with paraquat （100 mg/kg） .The rats in DG group were intragastriced administration with DG （50 mg/kg） once per day after paraquat injection.Pulmonary lung wet to dry weight ratio was determined;the lung tissue HE staining was used to evaluate the pulmonary tissue injury;to detect the expression of lung tissue TLR -4 mRNA and NF -κB mRNA RT -PCR method was used.The concentration of IL-6 and IL-17 in serum were determined by ELISA.Results Compared with the control group, the lung tissue＆#39;s wet/dry weight ratio, expression of lung tissue TLR-4 mRNA, NF-κB mRNA, IL-6 and IL -17 in serum increased significantly and comparison between groups showed statistical significance （P〈0.01 or P〈0.05）.7 day mortality was 90% in PQ group, 40% in DG group, and no death in NS group.Conclusion DG can attenuate the acute lung injury induced by paraquat poisoning in rats, the mechanism may be related to the decrease of TLR -4 and NF -κB mRNA expression.

关 键 词：甘草酸二铵(DG) 百草枯(PQ) Toll样受体4(TLR-4) 急性肺损伤(ALI) 

分 类 号：R734.2[医药卫生—肿瘤]