肝细胞生长因子对重症急性胰腺炎小鼠肠黏膜屏障保护作用  

Protective effect of hepatocyte growth factor on intestinal mucosal barrier following acute pancreatitis in mice

作　　者：孙善林[1] 张宗兵[2] 孔令尚[2] 刘牧林[2] 汪华学[3] 何先弟[3] 姜从桥[2] 刘瑞林[2] 

机构地区：[1]蚌埠医学院胃肠外科,安徽省233030 [2]蚌埠医学院第一附属医院胃肠外科 [3]蚌埠医学院第一附属医院重症医学科

出　　处：《中华解剖与临床杂志》2014年第5期427-430,共4页Chinese Journal of Anatomy and Clinics

基　　金：安徽省卫生厅科研资助项目（2008B009）

摘　　要：目的探讨肝细胞生长因子（HGF）对重症急性胰腺炎（SAP）小鼠肠黏膜屏障功能障碍修复作用及机制，以及肠道干细胞在黏膜损伤修复中的作用。方法健康雄性昆明小鼠60只，按数字表法随机分成正常对照（NC）组10只、SAP组25只和HGF组25只。酶耦联紫外分光光度法检测血清D哥L酸水平，肠系膜淋巴结、胰腺、肝脏、脾脏及肺组织细菌培养，流式细胞仪检测肠上皮细胞增殖指数，免疫组化染色法检测小肠隐窝干细胞变化。结果SAP组血清D-乳酸值为（11．17±1．90）mg／L，较NC组（4．87±0．73）mg／L和HGF组（7．30±0．88）mg／L高，差异均有统计学意义（F=78．20，P值均〈0．01）。胰腺、肠系膜淋巴结、肝脏、脾脏、肺脏细菌培养器官移位率SAP组（50％，40／80）高与NC组（14％，7／50）和HGF组（29％，29／100）（χ^2=7．427，χ^2=4．112，P值均〈0．01）。肠黏膜上皮细胞增殖指数SAP组较NC组和HGF组明显下降（F=42．71，P值均〈0．05），HGF组与NC组比较则明显升高（P〈0．01）。SAP组小肠隐窝干细胞数（1．26±0．87）个，低于NC组（2．16±0．90）个和HGF组（2．50±0．96）个，差异均有统计学意义（F=8．34，P值均〈0．01）。结论HGF可促进肠黏膜上皮细胞的增殖，降低肠道通透性及肠道细菌移位率，减轻急性胰腺炎小鼠肠黏膜的损伤。Objective To investigate the repair mechanism of hepatocyte growth factor （HGF） on intestinal mucosal barrier dysfunction of the severe acute pancreatitis （SAP） mice, and to evaluate the effect of intestinal stem cell on the repair mechanism of intestinal mucosal barrier dysfunction. Methods All healthy male Kunming mice were divided into normal control （n = 10）, SAP （n =25） and HGF group （n = 25） by random location. Serum D-lactate was deteted by automatic biochemistry analyzer and ultraviolet spectrophomotometry. The tissues of mesenteric lymph nodes, pancreas, liver, spleen and lung were cultured to determined baceterial tranlocation. Intestinal mucosal epithelial cells proliferation index（PI） was analyzed by flow cytometry （FCM）. The changes of intestinal stem cells in the crypt were observed by immunohistochemistry. Results Compared to NC group （4.87 ±0.73 ） mg/L and HGF group （7.30± 0.88） mg/L, serum D-lactate was significantly increased in SAP group（ 11.17 ± 1.90） mg/L（ F = 78.20, all P values 〈 0.01 ）. In comparison with NC group （14%, 7/50）, organ bacterial translocation in tissues of mesteric lymph nodes, pancreas, liver, spleen and lung were significantly increased in SAP group（50%, 40/80, χ^2 =7.427, P 〈0.01）, but was lower in HGF group （29%, 29/100） than those in SAP group （50%, 40/80, χ^2 = 4. 112, P 〈 0.01 ）. Compared to NC group, PI was significantly decreased in SAP group（P 〈0. 01 ）, but was higher in HGF group than those in SAP group, and was higher in HGF group than those in NC group. Compared to NC group （2. 16 ± 0.90）, intestinal stem cell was significantly decreased in SAP（ 1.26 ±0.87）, and was higher in HGF group （2.50 ± 0.96） than those in SAP （F = 8. 34, all P values 〈 0.05 ）. Conclusions HGF can not only significantly reduce the injury of intestinal mucosa in mice with acute pancreatitis but can also promote the proliferation of intestinal mucosal epithelial cells. Intestinal mucosal permeab

关 键 词：急性胰腺炎 肠屏障 肝细胞生长因子 干细胞 

分 类 号：R576[医药卫生—消化系统]