热休克蛋白72-甲胎蛋白抗原表位肽复合物抗小鼠肝癌Hepal-6细胞免疫的实验研究  

Anti-tumor immunity induced by recombinant peptides complex- heat shock protein 72 and alpha-fetoprotein epitope peptide

作　　者：胥冰 王小平 马晓军 张可佩 赵倩 任梦凡 房景刚 张景慧 

机构地区：[1]陕西中医学院分子病理学研究室,陕西西安712046

出　　处：《现代肿瘤医学》2014年第11期2528-2531,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(No.81172135);陕西省教育厅自然科学基础研究项目(No.07JK233)

摘　　要：目的:以热休克蛋白72(HSP72)-甲胎蛋白(AFP)抗原表位肽复合物免疫小鼠,研究该复合物针对AFP肿瘤是否具有特异性抗肿瘤免疫。方法:以HSP72-AFP多肽复合物皮下注射免疫昆明小鼠,并分别以AFP多肽和HSP72单独免疫小鼠为对照组。ELISA法检测免疫后小鼠血清IFN-γ水平、MTT法检测各免疫组小鼠淋巴细胞对肝癌Hepal-6细胞的杀伤作用、以小鼠体内瘤负荷实验评价蛋白复合物的免疫效应。结果:HSP72-AFP多肽复合物组小鼠血清IFN-γ水平、淋巴细胞对Hepal-6细胞的杀伤作用均显著高于AFP多肽和HSP72组(P<0.01)。HSP72-AFP多肽复合物组瘤体积也明显小于AFP多肽和HSP72免疫组(P<0.01)。结论:HSP72-AFP多肽复合物疫苗可诱导荷瘤小鼠产生针对AFP肿瘤的特异性细胞免疫,其对瘤细胞的杀伤效应明显优于两者单一的多肽纯化疫苗。表明HSP72-AFP多肽复合物可诱导小鼠产生有效的抗肿瘤免疫。Objective：To construct a recombinant peptides complex-alpha-fetoprotein （AFP） and heat shock protein 72 （HSP72）,and study its ability to induce specific CTL response and its protective effect against AFP-producing tumor.Methods：A recombinant peptides complex was constructed by conjugating mouse alpha-fetoprotein （mAFP） with heat shock protein 72 protein.Mice were primed and boosted with recombinant peptides complex HSP72-AFP,single AFP or HSP72 as controls.ELISA was used to test IFN-γ-producing splenocytes and the level of serum anti-AFP antibody from immunized mice.Results：By recombinant HSP7 2-AFP peptides complex immunization,the results of ELISPOT and ELISA showed that the number of splenic cells producing IFN-γand the level of anti-AFP antibody of serum were significantly higher in HSP72-AFP group than those in AFP and HSP72 groups （P 〈0.01）.The tumor volume in HSP72-AFP group was significantly smaller than that in AFP and HSP72 groups （P 〈 0.01）.Conclusion：Sequential immunization with recombinant HSP72-AFP peptides complex could generate effective AFP-specific T cell responses and induce definite antitumor effects on AFP-producing tumors,which may be suitable for some clinical testing as a vaccine for HCC.

关 键 词：甲胎蛋白(AFP)抗原表位肽 热休克蛋白72 (HSP72) 复合物 肿瘤免疫 

分 类 号：R73-362[医药卫生—肿瘤] R735.7[医药卫生—临床医学]