Survivin shRNA与大黄素联合应用抗人卵巢癌细胞株增殖的实验研究  

The study of anti-prolifetion effect by combination of survivin shRNA and emodin in human ovarian cancer cell line

作　　者：薛晖[1] 李天人[1] 贺安宁[2] 郭科军[1] 

机构地区：[1]中国医科大学附属第一医院妇科,辽宁沈阳110001 [2]中国医科大学肿瘤研究所第一研究室,辽宁沈阳110001

出　　处：《现代肿瘤医学》2014年第11期2557-2561,共5页Journal of Modern Oncology

摘　　要：目的:探讨靶向survivin基因的shRNA与大黄素联合应用体外抗人卵巢癌细胞株SKOV3和HO8910增殖的作用。方法:构建携带靶向survivin基因的shRNA质粒载体,脂质体法转染。MTT法检测survivin shRNA质粒转染或/和大黄素处理后SKOV3和HO8910细胞的增殖率,FCM法检测细胞凋亡情况。结果:Survivin shRNA质粒转染24h后SKOV3和HO8910细胞的增殖率下降,凋亡率增加,与空白对照组差别均有统计学意义(P<0.05);大黄素对SKOV3和HO8910细胞的抗增殖作用呈浓度和时间依赖模式;survivin shRNA与大黄素(60μmol/L)联合应用组SKOV3和HO8910细胞的增殖率低于单药应用组(P<0.05),凋亡率高于单药应用组(P<0.05)。结论:Survivin shRNA与大黄素联合应用能够增强抗人卵巢癌细胞株SKOV3和HO8910的体外增殖作用。Objective：To investigate the efficacy of a strategy combining survivin targeted short hairpin RNA （shRNA） with emodin on anti-proliferation of human ovarian cancer cell line SKOV3 and HO8910.Methods：Plasmid vector with survivin shRNA was constructed and transfected into human ovarian cancer cell line SKOV3 and HO8910 through liposome method.Expression levels of survivin mRNA were assayed by real time PCR and the protein expression levels were assayed by Western blot technology.The combined effect of survivin shRNA and emodin on cells viability were detected by MTT assay.And FCM was applied to study the apoptosis induction effect of survivin shRNA and emodin.Results：The expression of survivin mRNA decreased obviously in SKOV3 and HO8910 cells after transfection,which significantly different from the blank control group （P 〈 0.05）.Survivin protein expression level decreased in accordance with the results of real time PCR.Transfection of survivin shRNA plasmid can effectively decrease the proliferation rates of SKOV3 and HO8910 cells,which were obviously different from the blank control group （P 〈0.05）.Emodin can inhibit the growth of SKOV3 and HO8910 cells in a concentration-and time-dependent model.The combination of survivin shRNA and emodin （60μmol/L） can inhibit the proliferation of SKOV3 and HO8910 cells and increase the apoptosis rates more effectively,which was different from single-agent applications group significantly （P 〈 0.05）.The apoptosis rate of cells transfected with survivin shRNA was higher than the control group（P 〈 0.05）.Apoptosis rates induced by the combination of the two agents can further increase the apoptosis rates of SKOV3 and HO8910 cells,which obviously higher than single drug application group （P 〈 0.05）.Conclusion：Both transfection of survivin shRNA plasmid and emodin（60μmoL/L） could inhibit the proliferation and induce apoptosis of human ovarian cancer cell line SKOV3 and HO8910,and these effects were enhanced by combination of surv

关 键 词：卵巢肿瘤 SURVIVIN SHRNA 大黄素 增殖 凋亡 

分 类 号：R737.31[医药卫生—肿瘤]