DNA依赖蛋白激酶在人喉鳞状细胞癌中的表达及意义  

Expression and significance of DNA-dependent protein kinase in human laryngeal squamous cell carcinoma

作　　者：孙建松[1] 杨秀海[1] 姬红培[1] 赵睿[1] 代喻兵[1] 

机构地区：[1]贵州省人民医院耳鼻咽喉头颈外科,贵阳550002

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第21期1641-1644,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：贵州省科技联合基金[No:黔科合LS字(2011)016号]

摘　　要：目的:研究DNA依赖蛋白激酶(DNA-PK)在人喉鳞状细胞癌(LSCC)及正常喉黏膜组织中的表达,探讨其与LSCC临床病理特征之间的关系。方法:应用免疫组织化学方法(Envision)检测64例LSCC组织(实验组)及15例正常喉黏膜组织(对照组)中DNA-PK的表达水平,结合LSCC的临床病理特征进行分析。结果:DNA-PK在对照组和实验组中的阳性表达率分别为26.67%(4/15)、78.13%(50/64),差异有统计学意义(P<0.05)。DNA-PK表达与LSCC的组织分化程度相关(P<0.05),与临床分期、淋巴结转移无明显相关(P>0.05)。结论:DNA-PK的表达与LSCC的病变发展有关。Objective：To study the expression of DNA-dependent protein kinase（DNA-PK）in human laryngeal squamous cell carcinoma（LSCC）and normal laryngeal mucosa（NLM）,and to analysize the relationship between the expression and the clinicopathologic parameters of LSCC.Method：Immunohistochemical technique（Envision）was used to detect the expression of DNA-PK in 64 cases of LSCC and 15 cases of NLM.To investigateAn investigation was conducted on the relationship between the expression and clinico-pathological features of LSCC.Result：DNA-PK was lowly expressed in NLM and highly expressed in LSCC,the positive rate of DNA-PK expression was 26.67%（4/15）,78.13%（50/64）,respectively,and there was significant different difference between the two groups（P〈0.05）.Its expression was correlated with the level of histodifferentiation（P〈0.05）,but not with TNM stages and neck lymph node metastasis（P〈0.05）.Conclusion：DNA-PK may be involved in disease development of LSCC.

关 键 词：喉黏膜 喉鳞状细胞癌 DNA依赖蛋白激酶 免疫组织化学 

分 类 号：R739.6[医药卫生—肿瘤]