减阻牵张快速牙移动中TGF-β1表达变化对牙周组织改建的影响  

Effect of TGF-β1 expression on periodontal tissue reconstruction for rapid teeth movement through reducing resistance and distraction

作　　者：李宁 杨乐乐[2] 彭早霞[3] 李佩[2] 李美静[2] 陈曦[2] 徐然[2] 

机构地区：[1]烟台市口腔医院正畸科,山东烟台264000 [2]西安交通大学医学院第一附属医院口腔科,陕西西安710061 [3]新疆医科大学第一附属医院口腔科,新疆乌鲁木齐830000

出　　处：《上海口腔医学》2014年第5期513-518,共6页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81070863);陕西省科技攻关项目(2014k11-03-08-02)~~

摘　　要：目的:探讨减阻牵张快速牙移动中转化生长因子β1(TGF-β1)在牙周组织改建中的作用。方法:20只Beagle犬随机均分为加力5、10、15 d、加力15 d保持10、90 d 5组。于下颌骨两侧随机分别采用减阻牵张(实验组)和常规正畸方法(对照组)移动第一前磨牙。各组犬分别于预定时间测量牙移动距离并获取第一前磨牙牙周组织块,进行H-E、苦味酸-天狼星红、免疫组化染色观察。采用SPSS 18.0软件包对数据进行统计学分析。结果:实验组牙移动及牙周改建速度显著快于对照组;2组TGF-β1阳性表达分布区域相似,均于15 d骨生成最活跃阶段达到峰值,但加力各阶段实验组阳性表达显著高于对照组(P<0.05)。结论:与常规正畸方法相比,减阻牵张能明显增加移动牙张力侧牙周组织TGF-β1的表达,加速牙周组织改建。PURPOSE： To investigate the role of transforming growth factor-β1 （TGF-β1） in periodontal tissue reconstruction during rapid teeth movement through reducing resistance and distraction. METHODS： Twenty Beagle dogs were randomly divided into five groups according to time points： distraction for 5 days, 10 days, 15 days, retaining 10 days after distraction for 15 days, and retaining 90 days after distraction for 15 days. The mandibular first premolar was moved by using reducing resistance and distraction （experimental group） or conventional distraction （control group） randomly. For each group, tooth movement distance was measured and first premolar periodontal tissue pieces were obtained in scheduled time. Hematoxylin-eosin stain, Picric acid-Sirius red, and immunohistoehemistry were performed. The data was analyzed by using SPSS 18.0 software package. RESULTS： The speed of tooth movement and periodontal reconstruction of the experimental group were significantly faster than the control group. TGF-β1 positive expression in two groups had similar distribution area, and reached peak at most active stage of osteogenesis in both groups. But in different stages of distraction, the positive expression in the experimental group was significantly higher than the control group （P〈0.05）. CONCLUSIONS： Compared with conventional orthodontic method, reducing resistance and distraction can obviously increase the expression of TGF-β1 of tension side of the distracted tooth, and accelerate periodontal tissue reconstruction.Supported by National Natural Scinece Foundation of China （81070863） and Science and Technology Project of Shaanxi Province （2014k11-03-08-02 ）.

关 键 词：牵张成骨 快速牙移动 转化生长因子Β1 

分 类 号：R783.5[医药卫生—口腔医学]