Mus81对人乳腺癌SKBR3增殖、侵袭和迁移的影响  

Effects of Mus81 on Proliferation,Invasion and Migration of Human Breast Cancer SKBR3

作　　者：吴云路[1] 钱颖[1] 吕娟[2] 严秋月[1] 董学君[1,2] 

机构地区：[1]温州医科大学检验医学院,生命科学学院,325035 [2]浙江省绍兴市人民医院,浙江大学绍兴医院临床检验中心

出　　处：《医学研究杂志》2014年第10期58-63,共6页Journal of Medical Research

基　　金：浙江省重点科技创新团队(2012R10048-09);浙江省医药卫生平台重点基金资助项目(2013ZDA024)

摘　　要：目的研究Mus81过表达对人乳腺癌SKBR3增殖、侵袭和迁移能力的影响。方法从相应甘油菌中抽提Mus81过表达质粒和阴性对照质粒,通过Lipofectamine2000脂质体转染人乳腺癌SKBR3细胞。分别利用RT-PCR和Western blot法检测Mus81过表达效果,CCK-8增殖实验检测过表达前后细胞增殖能力变化,Transwell侵袭实验检测过表达前后细胞侵袭能力变化,Transwell迁移实验和细胞划痕实验检测过表达前后细胞迁移能力变化。结果 RT-PCR和Western blot法表明Mus81基因过表达效果良好。Mus81过表达后,增殖实验结果显示实验组细胞增殖速度明显低于对照组(P<0.05)。Transwell侵袭实验显示,实验组细胞每高倍视野平均穿膜个数低于对照组(P<0.05)。Transwell迁移实验显示,实验组细胞每高倍视野平均穿膜个数同对照组相比,差异无统计学意义(P>0.05)。细胞划痕实验结果显示,实验组划痕愈合率同对照组相比,差异无统计学意义(P>0.05)。结论 Mus81过表达抑制了人乳腺癌SKBR3细胞的增殖,降低了细胞的侵袭能力,但对SKBR3细胞迁移能力没有明显影响。Objective To investigate the effects of Mus81 overexpression on proliferation,invasion and migration ability of human breast cancer SKBR3 cells.Methods SKBR3 breast cancer cells were transfected using Lipofectamine（R） 2000 with the plasmids containing over-expression Mus81 or negative ones from the glycerin bacteria.The overexpression of Mus81 gene was determined by RT-PCR and Western blot.Changes in cell proliferation was detected by CCK-8 proliferation assay.The changes of invasion and migration ability before and after transfection were evaluated using Transwell invasion assay,Transwell migration assay and wound assay.Results The results of RT-PCR and Western blot confirmed Mus81 gene overexpressed successfully.The proliferation rate of the experimental group was significantly lower than the control group （P 〈 0.05）.Transwell invasion assay indicated that the experimental group exhibited a decrease in the number of cells invading the lower chamber,compared to the control group（P 〈 0.05）.Transwell migration assay and wound assay did not displayed statistically significant difference.Conclusion Mus81 overexpression inhibited the proliferation and decreased invasion ability of human breast cancer SKBR3 cells,but had no significant effect on SKBR3 cell migration.

关 键 词：Mus81 乳腺癌 增殖 侵袭 迁移 

分 类 号：R737[医药卫生—肿瘤]