5-硝基愈创木酚钠对韭菜叶片硝酸还原同化的影响  

Effects of 5-Nitroguaiacol Sodium on Nitrate Reduction and Assimilation in the Leaves of Chinese Chive

作　　者：李红利[1] 王俊玲[2] 薛占军[1] 王梅[1] 高志奎[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]河北农业大学生命科学学院,河北保定071001

出　　处：《西北植物学报》2014年第10期2028-2033,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：河北省科技支撑计划(11220701D);河北省自然科学基金(C2013204076)

摘　　要：以3年生‘大金钩韭菜’为供试材料,采用10μmol·L-1的5-硝基愈创木酚钠(5-NGS)进行叶面喷施处理,分析对韭菜叶片硝酸盐含量、氮代谢关键酶活性、氨基酸组分、营养品质及叶绿素荧光动力学活性的影响,以探索5-NGS对促进NO3-还原同化的相关机制。结果表明:(1)5-NGS处理使韭菜叶片硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、谷氨酸草酰乙酸转氨酶(GOT)和谷氨酸丙酮酸转氨酶(GPT)等氮代谢关键酶活性分别比对照显著提高58.7%、26.5%、179.4%、131.3%,硝酸盐含量显著降低26.1%。(2)5-NGS处理韭菜叶片的可溶性蛋白、维生素C分别显著提高49.5%、25.0%,氨基酸总量及其组分含量也显著增加,但可溶性糖含量显著降低。(3)5-NGS处理使韭菜叶片的PSⅡ电子传递速率显著增加11.3%,其生物量、叶面积、叶绿素含量等也显著增加。研究认为,韭菜叶面喷施5-NGS使得末端的蛋白质合成活性增强,拉动了游离氨基酸向蛋白质的转化,同时调动了转氨作用的积极协同配合,还可能调动碳同化产物的协同配合,促进了前端硝态氮转化为谷氨酸和衍生氨基酸,从而降低了韭菜叶片的NO3-累积,并改善营养品质,促进了植株生长。The 5-nitroguaiacol sodium （5-NGS, 10 μmol·L-1） was applied to spray 10cm height of Chinese chives （cv. Dajingou）. The nitrate content, key enzyme activities of nitrogen metabolism, amino acid compo nents, nutritional quality and chlorophyll a fluorescence were measured and compared with control treatment （spraying water） at 12 d. The results showed that： （1）5-NGS significantly enhanced activities of nitrate reductase（NR）, glufaminc synthctasc （GS）, glutamate oxaloacetate transaminase （GOT）, glutamate pyruvate transaminase（GPT） by 58.7%, 26.5%, 179.4%, 131.3%,and the nitrate content reduction by 26.1 %. （2）5-NGS significantly enhanced soluble protein, Vc contents by 49.5 %, 25.0 %, respectively. Sig- nificantly higher contents of 15 kinds of amino acids were also observed in 5-NGS-treated leaf than that in control leaf of Chinese chives. However, soluble sugar content in 5-NGS-treated leaf was lower than that in control leaf. （3） 5-NGS significantly enhanced biomass, leaf area, chlorophyll content, and increased electrontransport rate（ETR） by 11. 3%. Thus,the ability of 5-NGS to increase nitrate reductase（e, g. NR） and higher nitrogen assimilation enzyme（e, g. GS, GOT, GPT） activities might be an important attribution linked to low nitrate accumulation in leaves of Chinese chive.

关 键 词：5-硝基愈创木酚钠 韭菜 硝酸盐累积 氨基酸组分 

分 类 号：Q945.1[生物学—植物学]