慢性压迫背根神经节大鼠模型背根神经节Wnt5a的表达变化  

EXPRESSION OF Wnt5a IN DORSAL ROOT GANGLIA IN RAT MODEL OF CHRONIC COMPRESSION OF DORSAL ROOT GANGLIA

作　　者：曹振昊 田云虎[2] 马璐璐[1] 赵厚岭 

机构地区：[1]潍坊医学院研究生院,山东潍坊261042 [2]潍坊医学院附属医院脊柱外科

出　　处：《中国修复重建外科杂志》2014年第11期1392-1396,共5页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的观察Wnt5a在慢性压迫背根神经节(chronic compression of dorsal root ganglia,CCD)大鼠模型背根神经节的表达变化,探索Wnt5a在神经根病中的作用及神经根病中TNF-α与Wnt5a的关系。方法取192只成年雄性SD大鼠随机分为4组,每组48只,分别为假手术组(A组)、CCD组(B组)、CCD+生理盐水组(C组)、CCD+依那西普组(D组)。B、C、D组按照Hu等方法制备CCD模型后,C、D组分别腹腔注射生理盐水(5.5 mg/?kg)和依那西普(5.5 mg/kg);A组仅暴露椎间孔。各组于术后1、3、5、7 d利用von Frey纤维细丝检测大鼠后足机械痛阈值;术后3、7 d分别采用免疫组织化学染色和RT-PCR法检测背根神经节中Wnt5a蛋白和基因表达。结果术后各时间点B、C组机械痛阈值均显著低于A、D组,D组低于A组,差异均有统计学意义(P<0.05);B、C组间差异无统计学意义(P>0.05)。免疫组织化学染色示各组大鼠背根神经节的大、中、小神经元均有Wnt5a表达。除A组外,术后7 d B、C、D组Wnt5a阳性细胞数和Wnt5a mRNA相对表达量均明显高于术后3 d(P<0.05)。术后3、7 d,B、C组Wnt5a阳性细胞数和Wnt5a mRNA相对表达量显著高于A、D组,D组高于A组,差异均有统计学意义(P<0.05);B、C组间差异无统计学意义(P>0.05)。结论 CCD大鼠模型背根神经节中Wnt5a表达增加,阻断TNF-α后背根神经节Wnt5a表达减少。Objective To investigate the role of Wnt5a in the mechanism of radiculopathy and the relation between Wnt5a and tumor necrosis factor α （TNF-α） by observing the change of the expression of Wnt5a in the rat model of chronic compression of dorsal root ganglia （CCD）. Methods A total of 192 adult male Sprague Dawley rats were allocated into 4 groups： shame group （group A, n=48）, CCD group （group B, n=48）, CCD ＋ saline group （group C, n=48）, and CCD ＋etanercept group （group D, n=48）. An L-shaped needle （about 3.5 mm in length, 0.6 mm in diameter） was inserted into the L5 intervertebral foramen, and the dorsal root ganglia （DRG） was compressed by the needle to prepare the CCD model in groups B, C, and D, and then normal saline （5.5 mg/kg） or etanercept was injected intraperitoneally in groups C and D.The intervertebral foramen was exposed in group A. The mechanical pain threshold of the posterior paw was tested by the von Frey filaments at 1, 3, 5, and 7 days after operation; the expressions of Wnt5a protein and mRNA were detected at 3 and 7 days after operation by immunohistochemical staining and RT-PCR, respectively. Results The mechanical pain threshold of groups B and C was significantly lower than that of groups A and D, and in group D than in group A （P〈0.05）, but no significant difference was found between groups B and C （P〈0.05）. The Wnt5a positive cells and the mRNA expression of Wnt5a at 7 days were significantly more than those at 3 days in groups B, C, and D （P〈0.05）. The Wnt5a positive cells and the mRNA expression of Wnt5a in groups B and C were significantly more than in groups A and D, and in group D than in group A （P〈0.05）, but no significant difference was shown between groups B and C （P〈0.05）. Conclusion The expression of Wnt5a in the DRG is increased after CCD. The expression of Wnt5a in the DRG is decreased after the administration of the inhibitor of TNF-α.

关 键 词：背根神经节 WNT5A 慢性压迫背根神经节模型 神经根病 TNF-Α 大鼠 

分 类 号：R-332[医药卫生] R681.5