9个miniSTR基因座在西藏藏族人群中的遗传多态性  

Genetic polymorphism of 9 miniSTR loci in Tibetan population

作　　者：李成志[1] 任甫[1] 席焕久[1] 奇双 马庆云[1] 李文慧[1] 

机构地区：[1]辽宁医学院生物人类学研究所法医学教研室,锦州121001

出　　处：《解剖学杂志》2014年第5期682-686,共5页Chinese Journal of Anatomy

基　　金：国家自然科学基金（31171152）

摘　　要：目的：对西藏藏族人群的9个miniSTR基因座（D20S1082、D6S474、D12 ATA 63、D9S1122、D2S1776、D1S1627、D3S4529、D2S441、Amelogenin）进行多态性研究.方法：用Chelex-100法提取西藏藏族人群96份无关个体血液样本DNA,在Mastercylcer（R）pro梯度PCR仪上对9个miniSTR基因座进行扩增,运用3130基因分型仪检测并收集电泳结果,通过GeneMarkerV 2.2.0软件计算扩增产物片段相对大小以及进行样本基因型分型.结果：9个miniSTR基因座（D20S1082、D6S474、D12 ATA 63、D9S1122、D2S1776、D1S1627、D3S4529、D2S441、Amelogenin）检出等位基因分别为5、5、6、7、6、4、5、6、2,均符合Hardy-Weinberg平衡,杂合度在0.531～0.742之间,累计个人识别率为0.999 998,累计非父排除率为0.984 083.结论：该9个miniSTR基因座在西藏藏族人群中的多态性较好,个体识别率高,可应用于个体识别和亲权鉴定.Objective： To investigate the genetic polymorphism of 9 miniSTR loci （D20S1082, D6S474, D12ATA63, D9S1122, D2S1776, D1S1627, D3S4529, D2S441, Amelogenin） in Tibetan population. Methods： The DNA was extracted by Chelex-100 method from blood samples of 96 unrelated individuals in Tibetan population. Amplification of 9 miniSTR loci was performed on the gradient PCR. Data were collected by the 3130 gene analyzer. The length of the amplification fragments was calculated and the genotyping was made using GeneMarker V 2.2.0 software. Results： 9 miniSTR loci （D20S1082, E）6S474, D12ATA63, D9S1122, D2S1776, D1S1627, D3S4529, D2S441, Amelogenin） showed respectively 5, 5, 6, 7, 6, 4, 5, 6 and 2 alleles. All loci were in Hardy-Weinberg equilibrium. The heterozygosity of the 9 miniSTR loci was between 0. 531 and 0. 742. The total probability of discrimination power （TPD） was 0. 999 998 and the cumulate exclusion probability of paternity （CPE） was 0. 984 083. Conclusion： The 9 miniSTR loci show high genetic polymorphism and great discrimination power in Tibetan population. It can be used for personal identification and paternity testing.

关 键 词：法医物证学 遗传多态性 MINISTR 藏族 西藏 

分 类 号：R394[医药卫生—医学遗传学]