耐低温淀粉酶产生菌C-9的筛选、鉴定及酶学特性研究  

Study on isolation.identification and enzymatic of amylase C9 produced by psychrotolerant bacterial strain

作　　者：李清扬[1,2] 崔冠慧[1] 习彦花[1] 李欣[1] 张根伟[1] 程辉彩[1] 

机构地区：[1]河北省科学院生物研究所,河北石家庄050081 [2]河北农业大学食品科技学院,河北保定071001

出　　处：《中国饲料》2014年第21期18-20,共3页China Feed

基　　金：河北省科技支撑项目(13206621D);河北省财政预算项目(14334/14315)

摘　　要：采用Hungate厌氧操作技术，从东北地区土壤中分离到一株产耐低温淀粉酶菌株C-9，经形态、生理生化、16SrDNA序列分析，鉴定为丁酸梭菌（Clostridiumbutyricum）。该淀粉酶最适作用温度为30～40℃，属于低温淀粉酶。A psychrotolerant strain producing amylase was isolated from the soil of Northeast China by hungate roll-tube technique.The results showed that the strain was Clostridium butyricum based on morphological,physiological and biochemical characteristics,and phylogenetic analysis of 16S rDNA.The strain C-9 belonged to the psychrotolerant strain. The amylase of C-9 coundn＇t resistant the high temperature,the optimum cultural temperature was 30-40℃.

关 键 词：丁酸梭菌 淀粉酶 菌种鉴定 酶学特性 

分 类 号：S816.3[农业科学—饲料科学]