羊膜细胞可保护和修复缺血再灌注损伤小鼠脑组织细胞  

Amniotic cells protect and repair mouse brain cells following ischemia-reperfusion injury

作　　者：郑彦涛[1] 刘斌[1] Robert Lodato 李奇林[1] 蓝迪慧 洪小英[3] 鲜华[3] 

机构地区：[1]南方医科大学珠江医院急诊科,广东省广州市510280 [2]德克萨斯大学休斯敦医学中心,美国德克萨斯州休斯敦77030 [3]南方医科大学第三附属医院急诊科,广东省广州市510282

出　　处：《中国组织工程研究》2014年第37期6024-6028,共5页Chinese Journal of Tissue Engineering Research

基　　金：广东省自然科学基金(S2011010003061)~~

摘　　要：背景:羊膜细胞主要由羊膜上皮细胞和羊膜间充质细胞组成,均具有多分化潜能,可转化为神经元,且还有合成、释放生物活性物质和神经营养因子的功能。作者前期研究证实羊膜细胞移植入脑内后,能明显促进脑内神经元的再生。目的:探索羊膜细胞对小鼠缺血再灌注损伤脑细胞的作用。方法:将Balb/C小鼠通过夹闭双侧颈总动脉方法建立脑缺血再灌注损伤模型后,分离小鼠脑细胞。取孕鼠新鲜胎盘,分离羊膜细胞。将与羊膜细胞共培养的小鼠脑细胞作为实验组,以PBS培养的小鼠脑细胞作为对照组。结果与结论:实验组小鼠脑细胞活性较对照组明显增加(P<0.05)。培养24,72 h后实验组小鼠脑细胞坏死率较对照组差异无显著性意义(P>0.05),而培养48 h后实验组小鼠脑细胞坏死率较对照组明显降低(P<0.05)。实验组小鼠脑细胞中S期细胞数量增加,而对照组小鼠脑细胞中G1期细胞数量增加,S期细胞数量减少,但2组小鼠脑细胞中G2期细胞数量不变。说明羊膜细胞具有保护缺血再灌注损伤Balb/C小鼠脑细胞的作用,且能抑制其坏死和凋亡并促进其再生。BACKGROUND：Amniotic cells are mainly composed of amniotic epithelial cells and amniotic mesenchymal cells, which have multi-differentiation potential and can be transformed into neurons as wel as synthesize and release biological y active substances and neurotrophic factors. In preliminary studies, amniotic cells that are transplanted into the brain can significantly promote the regeneration of brain neurons. OBJECTIVE：To explore the role of amniotic cells in mouse brain cells after ischemia-reperfusion injury. METHODS：The model of cerebral ischemia-reperfusion injury was established in Babl/c mice using occlusion of bilateral common carotid arteries, and then brain cells were separated from mice. Amniotic cells were isolated from mouse placenta. Brain cells from Balb/C mice co-cultured with amniotic cells served as experimental group, and brain cells cultured with PBS as control group. RESULTS AND CONCLUSION：The viability of brain cells in the experimental group was significantly higher than that in the control group （P〈0.05）. There was no difference in necrotic rate of brain cells between the experimental and control groups after 24 and 72 hours co-culture （P〉0.05）;after 48 hours co-culture, however, the necrotic rate of brain cells was significantly lower in the experimental group than the control group （P〈0.05）. In cellcycle, the experiment group showed increased S phase cells;while, the control group exhibited increased G 1 phase cells and decreased S phase cells. G 2 phase cells had no changes in number in both two groups. Through the above results, amnion cells can be proved to protect and promote the regeneration of brain cells of Balb/C mice with ischemia-reperfusion injury, and inhibit cellnecrosis and apoptosis.

关 键 词：干细胞 移植 羊膜细胞 BALB/C小鼠 脑细胞 缺血再灌注 细胞周期 凋亡 坏死 广东省自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]