模拟4000m高住及抗炎活血解痉中药干预力竭运动大鼠肾细胞超微结构变化  

A Chinese herb for anti-inflammation, blood circulation promotion and spasmolysis improves renal ultrastructure of rats undergoing 4-week exhaustive exercise at simulated altitude of 4 000 meters

作　　者：林喜秀[1,2] 赵用强[1] 谢亚萍[1] 邱继旺[1] 罗自强[2] 瞿树林[3] 

机构地区：[1]湖南工业大学体育学院,湖南省株洲市412000 [2]中南大学基础医学院博士后流动站,湖南省长沙市410000 [3]湖南师范大学医学院,湖南省长沙市410012

出　　处：《中国组织工程研究》2014年第38期6146-6152,共7页Chinese Journal of Tissue Engineering Research

基　　金：中国博士后第53批面上项(2013M531794);湖南工业大学湖湘体育文化研究基地资助(13HXTYWHO4);湖南工业大学2013年校级教育教学改革研究项目(2013C43);2012年湖南省博士后日常经费资助(2012;11);2012中南大学博士后启动基金~~

摘　　要：背景:抗炎活血解痉中药具有修复肾脏组织,改善肾脏血液供应,降低肾小球毛细血管的通透性,增强肾小管的滤过功能的作用。目的:探讨模拟4 000 m高住及抗炎活血解痉中药对4周力竭运动大鼠肾细胞超微结构及α-平滑肌肌动蛋白表达的影响。方法:将大鼠在速度为10 m/min坡度为0的跑台上进行适应性跑15 min,筛选出32只能完成跑的大鼠,随机分为正常对照组、力竭运动组、高住力竭运动组和给药力竭运动组,每组8只。正常对照组不进行运动,其他3组均进行力竭运动。高住力竭运动组模拟4 000 m高住后再进行力竭运动;给药力竭运动组先给予抗炎活血解痉中药灌胃,再进行4周力竭运动。采用苏木精-伊红染色、透射电子显微镜观察各组大鼠肾细胞的超微结构,免疫组织化学法检测肾组织α-平滑肌肌动蛋白的表达。结果与结论:力竭运动组可出现慢性肾损伤,肾小球滤过膜3层超微结构破坏,肾小管间质大量纤维化改变。与力竭运动组相比,高住力竭运动组和给药力竭运动组大鼠的运动量(跑距)增强(P<0.05),肾超微结构的损伤有明显的改善,α-平滑肌肌动蛋白的表达显著下降(P<0.01)。结果证实,抗炎活血解痉中药灌胃与模拟4 000 m高住干预对4周力竭运动诱导的肾超微结构损伤起保护作用。BACKGROUND： Chinese herb for anti-inflammation, blood circulation promotion and spasmolysis can repair the kidney, supply renal blood, reduce the permeability of the glomerular capillaries, and enhance renal filtration function,OBJECTIVE： To explore the effects of a Chinese herb for anti-inflammation, blood circulation promotion and spasmolysis on ultrastructure of renal cells and a-smooth muscle actin in rats after 4-week exhaustive exercise living at simulated altitude of 4 000 meters. METHODS： Thirty-two rats which could complete 15-minute adaptive run on the treadmill at a speed of 10 m/rain were enrolled and randomized into normal control group, exhaustive exercise group, exhaustive exercise＋high-altitude group and exhaustive exercise＋Chinese herb group, eight rats in each group. Except the normal control group, rats in the other three groups were subjected to exhaustive exercise, living at simulated altitude of 4 000 meters followed by exhaustive exercise, and intragastric administration of Chinese herb followed by 4-week exhaustive exercise, respectively. The ultrastructure of renal cells in rats was observed by hematoxylin eosin staining and transmission electron microscope, and expression of a-smooth muscle actin detected by immunohistochemical method. RESULTS AND CONCLUSION： In the exhaustive exercise group, rats appeared to have chronic renal injury, the ultrastructure of glomerular filtration membrane was damaged and tubulointerstitial fibrosis occurred. As compared with the exhaustive exercise group, the movement distance of rats in the exhaustive exercise＋high-altitude group and exhaustive exercise＋Chinese herb group was extended （P 〈 0.05）, and the damaged ultrastructure of the kidney was more obviously improved; in addition, the expression of a-smooth muscle actin was significantly decreased （P 〈 0.01 ）. Results show that the Chinese herb for anti-inflammation, blood circulation promotion and spasmolysis combined with living at simulated altitude of 4 000 meters can pro

关 键 词：组织工程 成骨细胞 增殖 碱性磷酸酶 组织构建 力竭运动 抗炎活血解痉中药 模拟4 000 m高住 Α-平滑肌肌动蛋白 肾滤过屏障 肾间质纤维化 干预 肾功能 

分 类 号：R318[医药卫生—生物医学工程]