硫化氢抑制离体灌流大鼠急性心肌缺血所致心肌细胞凋亡研究  

Hydrogen sulfide inhibits cardiomyocyte apoptosis induced by acute myocardial ischemia in isolated perfused rat heart

作　　者：谢英花[1] 马燕山[2] 张楠[3] 张建新[3] 

机构地区：[1]河北科技大学药学系,河北石家庄050018 [2]石家庄市中医院放射科,河北石家庄050051 [3]河北省医学科学院,河北石家庄050021

出　　处：《中国药理学通报》2014年第11期1543-1547,共5页Chinese Pharmacological Bulletin

基　　金：河北省自然科学基金资助项目(No C2009001458);河北省应用基础研究计划重点基础研究项目(No 13967602D)

摘　　要：目的探讨硫化氢(H2S)能否抑制离体灌流大鼠急性心肌缺血所致细胞凋亡。方法应用Langendorff离体灌流大鼠心脏复制急性心肌缺血损伤模型,结扎冠状动脉,心肌缺血4 h。40只♂SD大鼠随机分为5组(n=8):假手术组(Sham),缺血组(Ischemia)和硫氢化钠(NaHS)5、10、20μmol·L-1组。HE染色分析左心室心肌细胞组织形态学变化,TUNEL方法检测细胞凋亡,Western blot检测各组caspase-3和Cyt-C的蛋白表达。结果离体心肌缺血4 h后,光镜下可见心肌较大范围局灶性病变,细胞呈凝固性或带状坏死;心肌细胞凋亡率及心肌组织中caspase-3和Cyt-C蛋白表达明显升高。经NaHS 2 h处理,NaHS组光镜下心肌细胞结构清晰,心肌病变的程度明显减轻,大鼠心肌组织Cyt-C蛋白表达明显低于Ischemia组;NaHS 10、20μmol·L-1组心肌细胞凋亡率及心肌组织caspase-3蛋白表达明显低于Ischemia组。结论 H2S可通过抑制细胞凋亡对离体灌流大鼠急性心肌缺血发挥一定保护作用。Aim To investigate whether hydrogen sul-fide （ H2 S ） inhibits cardiomyocyte apoptosis induced by acute myocardial ischemia in isolated perfused rat heart. Methods The myocardial ischemia injury model was replicated with Langendorff isolated perfused rat heart, and the left anterior descending coronary ar-tery was ligated for 4 h. 40 male SD rats were divided into five groups randomly： sham group, ischemia＆amp;nbsp;group, and NaHS groups （5,10,20μmol·L-1）. The segmental heart samples were used for HE staining. Cardiomyocyte apoptosis was detected with TUNEL as-say. The expressions of caspase-3 and Cyt-C in hearts were determined with Western blot analysis. Results Myocardial cells were found to show serious disorder and coagulated zonal necrosis under light microscope, the apoptotic rate of cardiomyocytes and the expression of caspase-3 and Cyt-C were significantly increased af-ter ischemia for 4h. Perfusion of NaHS resulted in more clear cell morphology and milder pathologic chan-ges of myocardiocytes according to the HE staining a-nalysis, and the significant decrease of expression of Cyt-C. After perfusion of 10,20 μmol·L-1 NaHS,the apoptotic rate of cardiomyocytes and the expression of caspase-3 were significantly decreased. Conclusion H2 S has certain protective effects on acute myocardial ischemic injury in isolated perfused rat heart via inhibi-ting cardiomyocyte apoptosis.

关 键 词：硫化氢 急性心肌缺血 大鼠 细胞凋亡 心肌保护 

分 类 号：R-332[医药卫生] R322.11