环境对转基因小鼠抑郁样行为的影响  

The influence of environment on depressive-like behavior of transgenic mice

作　　者：陈静[1] 李晗[1,2,3] 张晨[1] 王祖承[1] 崔东红[1,2,3] 

机构地区：[1]上海交通大学医学院附属精神卫生中心,上海200030 [2]上海市重性精神病重点实验室 [3]上海交通大学精神疾病转化医学研究重点实验室

出　　处：《中国神经精神疾病杂志》2014年第9期547-551,共5页Chinese Journal of Nervous and Mental Diseases

基　　金：上海市科技发展基金实验动物研究项目(编号:12140904200);国家自然科学基金(编号:81171266;81271481);上海市重性精神病重点实验室(编号:13dz2260500)

摘　　要：目的探讨环境因素对精神分裂症断裂基因1(disruptedinschizophrenia1,DISC1)转基因小鼠抑郁样行为的影响。方法 C57BL/6野生型新生小鼠及C57BL/6 LBD-DISC1转基因新生小鼠各16只,分别随机分为野生实验组、野生对照组和DISC1实验组、DISC1对照组,每组8只。实验组小鼠在哺乳期进行6 d母婴分离,成年早期给予21 d慢性不可预知轻度应激,采用糖水消耗实验及强迫游泳实验测定四组小鼠行为,并比较各组小鼠的体质量改变。结果应激和DISC1缺陷对小鼠糖水偏好度没有交互作用(P>0.05),DISC1实验组糖水偏好度低于其余三组(均P<0.01);应激和DISC1缺陷对小鼠强迫游泳实验的静止时间有交互作用(P=0.010),DISC1实验组小鼠强迫游泳的静止时间长于野生对照组和DISC1对照组(均P<0.01);应激和DISC1缺陷对体质量改变亦没有交互作用(P>0.05),但DISC1实验组低于其余三组(均P<0.01)。结论应激和DISC1缺陷共同作用导致小鼠出现抑郁样行为。Objective To investigate the influence of environment on despressive-like behavior in DISC1 trans- genic mice. Methods Sixteen C57BL/6 wild-type mice were randomly assigned into two groups, the WT-stress group and the WT-control group. Sixteen C57BL/6 LBD-DISC1 transgenic mice were also randomly assigned into 2 groups, the DISCl-stress group and the DISCl-control group. The WT-stress group and the DISCl-stress group received daily 3 h maternal separation for six consecutive days and chronic unpredictable mild stress for 21 consecutive days. The sucrose consumption and the forced swim test were used to evaluate depressive-like behaviors. The changes of bodyweight were measured. Results There was no interaction （P 〉0.05） between environment and gene associated with the preference for sucrose solution. However, the preference for sucrose solution was lower in the DISCl-stress group than in the rest 3 groups （P 〈0.01）. Interaction between environment and gene associated with the immobility time was significant （P = 0.010） in the forced swim test. The immobility time was longer in the DISCl-stress group than in the WT-control group （P 〈0.01）. The bodyweight was lower in the DISC 1-stress group than in the rest 3 groups （P 〈0.01）. But the interaction of environment and gene associated with bodyweight was not significant （P 〉0.05）. Conclusion The interaction of gene and environment can induce depressive -like behavior in mice.

关 键 词：母婴分离 慢性不可预知轻度应激 糖水消耗实验 强迫游泳实验 抑郁症 

分 类 号：R749.4[医药卫生—神经病学与精神病学]