miRNA-21-3p对小鼠肝细胞系BNL CL.2体积和增殖的影响  

Effects of miRNA-21-3p on mouse hepatic cell line BNL CL.2 cell size and proliferation

作　　者：赵莹莹[1] 周秋莲[2] 王菲[1] 宋阳[1] 杨玥凤 杨长青[1] 

机构地区：[1]同济大学附属同济医院消化内科,上海200065 [2]上海大学生命科学学院再生实验室,上海200444

出　　处：《同济大学学报（医学版）》2014年第4期7-11,共5页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金(81070343)

摘　　要：目的观察miRNA-21-3p对小鼠肝细胞系(BNL CL.2)体积和增殖的影响。方法小鼠肝细胞系BNL CL.2转染miRNA-21-3p模拟物和抑制物。DiO染膜法和CCK-8法测定miRNA-21-3p对小鼠肝细胞系体积和增殖的影响;荧光定量PCR法检测miRNA-21-3p以及干预该miRNA后与细胞周期相关的基因的表达情况。结果在小鼠肝细胞系,转染miRNA-21-3p的模拟物可以显著增加miRNA-21-3p的表达,转染miRNA-21-3p抑制剂可以显著降低miRNA-21-3p的表达(P<0.01)。干预miRNA-21-3p不影响细胞的体积。增加miRNA-21-3p的表达显著促进细胞增殖(P<0.01),而抑制miRNA-21-3p的表达可以显著降低细胞增殖(P<0.01)。干预miRNA-21-3p,检测细胞周期相关的基因的表达,发现上调该miRNA可以下调Cyclin E(P<0.05),上调Ki67、p15、p16和p18(P<0.05),且对于CDKN3、Orc6、MCM4和p57没有影响(P>0.05)。下调该miRNA对于以上基因均没有影响(P>0.05)。结论 miRNA-21-3p对于小鼠肝细胞系体积没有影响,但是可以调控增殖,且它对于增殖的调控效应是部分通过影响周期相关的基因实现。Objective To investigate the effects of microRNA-21-3p （miRNA-21-3p） on the cell size and proliferation of mouse hepatic cell line BNL CL. 2. Methods BNL CL. 2 cells were transfected with miRNA-21-3p mimics or inhibitor. Cell size was determined with DiO staining while cell proliferation was determined by CCK-8 assay. The expression level of miRNA-21-3p and cell cycle- related genes were detected by Real-Time PCR. Results In BNL CL. 2 cells, miRNA-21-3p mimics increased the expression of miRNA-21-3p, while miRNA-21-3p inhibitor decreased the expression （P 〈 0.01 ）. Although miRNA-21-3p mimics significant increased proliferation of BNL CL. 2 cells, the cell size was not changed;while miRNA-21-3p inhibitors suppressed cell proliferation, miRNA-21-3p mimics significantly down-regulated Cyclin E expression（ P 〈 0.05 ） and up-regulated Ki67, p15, p16 and p18 （ P 〈 0.05 ） expression; while it did not affect CDKN3, Orc6, MCM4 and p57 expression （ P 〉 0.05 ）. The expression of cell cycle-related genes in BNL CL. 2 cells was not changed after transfected with miRNA-21-3p inhibitors （ P 〉 0. 05 ）. Conclusion miRNA-21-3p promotes proliferation of mouse hepatic BNL CL. 2 cells without affecting cell size, which is associated with regulating some cell cycle- related genes expression.

关 键 词：miRNA-21-3p 肝细胞 增殖 

分 类 号：R575.3[医药卫生—消化系统]