浙江省嘉兴地区健康和宫颈病变妇女人乳头瘤病毒感染情况分析  

Human papillomavirus infection of women with or without cervical lesions in Jiaxing in Zhejiang province

作　　者：俞春松 江敏敏 刘小香[2] 孙颜红[2] 孙爱华[2] 蒋锦琴[2] 张佳[3] 

机构地区：[1]嘉善县第一人民医院,314100 [2]浙江医学高等专科学校 [3]温州医学院检验医学院和生命科学学院

出　　处：《中华实验和临床病毒学杂志》2014年第5期339-341,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：浙江省卫生高层次创新人才培养工程项目（2012-141）;浙江省公益性技术应用研究计划项目（2013C37016）

摘　　要：目的 分析浙江省嘉兴地区宫颈病变及宫颈细胞学正常的妇女宫颈部位人乳头瘤病毒（HPV）感染率及基因型分布特点.方法 收集嘉兴地区4家医院经液基细胞学检查诊断为宫颈病患者426例,并选择液基细胞学检查宫颈细胞学正常的3134例同期健康体检妇女为对照,采用导流杂交法（HybriMax）对所有研究对象进行26种HPV基因型分型检测,比较有病变者与无病变者之间HPV感染率及其26种基因型的检出情况.结果 HPV DNA阳性检出率宫颈病变组（26.29％）明显高于健康体检组（11.35％）（P＜0.01）.HPV重叠感染率宫颈病变组（25.89％）明显高于宫颈细胞学正常的健康体检妇女（4.78％）（P＜0.01）.宫颈炎患者和CIN患者分别分离到的16种HPV高危型（HR-HPV） 63株（76.83％）和15种59株（83.10％） HR-HPV,CIN患者HR-HPV毒株感染率高于宫颈炎患者,但差异无统计学意义（P＞0.05）.宫颈炎患者HR-HPV构成比居前五位的分别是HPV16、33、58和18、52;CIN患者HR-HPV构成比居前五位的分别是HPV16、52和33、58、68.结论 本地区宫颈病变妇女宫颈部位HPV感染率及重叠感染情况明显高于宫颈细胞学正常的妇女,重叠感染与宫颈疾病发生相关,HPV-16、58、33和52是本地区宫颈病变妇女主要感染的高危型HPV.Objective To survey prevalence of human papillomavirus （HPV） and their distribution characteristics in women with and without cervical lesions in Jiaxing,Zhejiang province.Methods The flow-through hybridization technique （HybriMax） was used to detect HPV genotypes in 426 cases of women with cervical lesions and 3134 women with normal cytology.Samples were collected from 4 hospitals in Jinhua,Zhejiang province.HPV infection rate and 26 kinds of genotype detection rate were compared in women with and without cervical lesions.Results HPV prevalences in 426 women with cervical lesions （26.29%） was significantly higher than that of 3134 women without cervical lesions （11.35 %,P 〈 0.01）.HPV multiple infection rate of in HPV-positive women with cervical lesions （25.89%） was significantly higher than that of HPV-positive women without cervical lesions （4.78%） （P 〈 0.01）.High-risk HPV （HR-HPV） positive rate in cervicitis and cervical intraepithelial neoplasia （CIN） patients were 76.83%and 83.10%,respectively.CIN patients （83.10%） HR-HPV positive rate was higher than that of cervicitis patients （76.83%）,but there was no significant difference.The five most common prevalent high-risk HPVs were HPV-16,33,58 and 18,52 in cervicitis patients,and HPV16,52 and 33,58,68 in CIN patients.Conclusion HPV prevalences in women with cervical lesions were higher than those of normal cytology women （P 〈 0.01）.Multiple infection with HPV was associated with cervical lesions.HPV-16,HPV-58,HPV-33 and HPV-52 were the most common prevalent high-risk HPV in HPV-positive women with cervical lesions.

关 键 词：乳头状瘤病毒 人 宫颈疾病 宫颈炎 宫颈上皮内瘤样病变 基因型 

分 类 号：R737.33[医药卫生—肿瘤]