EBV-LMP2A和β-catenin蛋白在鼻咽癌中的表达关系及意义  

Relationship between the expression of EBV-LMP2A and β-catenin protein and their significance in nasopharyngeal carcinoma

作　　者：赵利容[1,2] 饶洁[1] 王艳 罗泊涛[1] 陈小毅[1] 

机构地区：[1]广东医学院病理学教研室,湛江524023 [2]四川省宜宾卫生学校,宜宾644000 [3]广东省深圳市龙华新区人民医院,深圳518000

出　　处：《临床与实验病理学杂志》2014年第10期1107-1112,共6页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家重点基础研究发展计划(973计划)子项目(2011CB504800)

摘　　要：目的探讨EB病毒编码的潜伏膜蛋白2A(latent membrane protein 2A,LMP2A)和β-连环素(β-catenin)在鼻咽癌(nasopharyngeal carcinoma,NPC)中的表达关系及其临床意义。方法应用免疫组化SP法检测LMP2A和β-catenin蛋白在32例鼻咽黏膜慢性炎、56例NPC和18例NPC淋巴结转移灶中的表达,采用Western blot法和免疫荧光细胞化学染色法观察LMP2A和β-catenin蛋白在CNE2-LMP2A细胞及其对照组CNE2-Vector和CNE2细胞中的表达。结果 (1)LMP2A在NPC及其淋巴结转移灶中的阳性率分别为89.3%(50/56)和77.8%(14/18),均明显高于鼻咽黏膜慢性炎(37.5%,12/32)(P均<0.01);β-catenin在NPC及其淋巴结转移灶中的异常表达率分别为62.5%(35/56)和83.3%(15/18),均明显高于鼻咽黏膜慢性炎(3.1%,1/32)(P均<0.01);LMP2A与β-catenin正常表达呈负相关(rs=-0.391,P<0.01);LMP2A和β-catenin蛋白在鼻咽癌T分期、N分期和临床分期组间表达率的差异均有统计学意义(P<0.05或P<0.01)。(2)CNE2-LMP2A细胞中β-catenin蛋白表达水平明显高于CNE2-Vector和CNE2细胞;CNE2-LMP2A细胞中β-catenin胞质和胞核的表达水平明显高于CNE2-Vector和CNE2(P均<0.01)。结论 LMP2A高表达和β-catenin异常表达与鼻咽癌临床生物学行为进展密切相关,其机制可能与LMP2A上调β-catenin蛋白水平并促进其核转位有关。Purpose To investigate the relationship between EBV-LMP2A and β-catenin and their clinical significance in nasopharyn- geal carcinoma （NPC）. Methods Immunohistochemistry was used to analyze the expression of LMP2A and β-catenin protein in 32 cases of nasopharyngitis, 56 cases of nasopharyngeal carcinoma and 18 cases of NPC lymph node metastasis. Then the protein expres- sion level of LMP2A and β-catenin in nasopharyngeal carcinoma cell line CNE2 with stablly expressing LMP2A （CNE2-LMP2A） , CNE2-Vector and CNE2 were detected by fluorescent immunocytochemistry and Western blotting, respectively. Results （ 1 ） Positive expression rate of LMP2A in nasopharyngeal carcinoma and its lymph node metastases was 89, 3 % （50/56） and 77.8% （ 14/18）, re- spectively, significantly higher than that in nasopharyngitis, 37. 5% （12/32） （ P 〈 0. 01 ）. The abnormal expression of β-catenin in nasopharyngeal carcinoma and lymph node metastasis was 62. 5% （35/56） and 83.3 % （15/18）, also significantly higher than that in nasopharyngitis, 3.1% （1/32） （P 〈 0. 01 ）. Spearman correlation analysis showed that LMP2A was negatively correlated with β- catenin in normal expression （ rs = -0. 391, P 〈0. 01 ）. The protein expression of LMP2A and β-catenin was positively related with T classification （T1 ＋ T2 vs T3 ＋ T4）, N classification （ NO ＋ N1 vs N2 ＋ N3 ） and clinical classification of NPCs （ P 〈 0. 05 or P 〈 0.01 ）. （2） The result of Western blot showed that the expression of LMP2A and β-catenin in CNE2-LMP2A was significantly higher than that in CNE2-vector and CNE2. The result of fluorescent immunocytochemistry showed that in the group of DMSO control, the ex- pression of β-catenin in CNE2-LMP2A was stronger than that in CNE2-vector and CNE2. In all of them,β-catenin mainly expressed in the membrane and cytoplasm but not in nuclei. By contrast, the experimental group which added MG132 showed that the β-catenin ex- pressed in the nucleus with differe

关 键 词：鼻咽肿瘤 潜伏膜蛋白2A Β-连环素 

分 类 号：R739.62[医药卫生—肿瘤]