TRAMP小鼠前列腺癌发生的病理进程观察  

Pathogenesis of prostate carcinoma in TRAMP mouse

作　　者：刘红英[1] 章倩倩[1] 周大磊[1] 雷岩[1] 胡曦文[1] 何晓东[1] 张钰[2] 王丽京[1] 

机构地区：[1]广东药学院/血管生物学研究所,广州510006 [2]广东省实验动物监测所/广东省实验动物重点实验室,广东510663

出　　处：《临床与实验病理学杂志》2014年第10期1144-1147,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(31271455;31200896;31100852);国家科技部"973"项目国家重点基础研究发展计划(2010CB529702)

摘　　要：目的探讨不同周龄转基因前列腺癌小鼠模型（transgenic adenocarcinoma of mouse prostate，TRAMP）的病理学特点。方法TRAMP小鼠饲养于SPF级环境中，记录其生存周期；通过组织病理学切片、HE染色及免疫组化法观察不同周龄TRAMP小鼠前列腺的病理学变化、SV40Tantigen（Tag）表达及细胞增殖。结果TRAMP小鼠普遍生存周期为30～55周，随着周龄的增加其前列腺异型增生程度愈加明显，16周左右处于明显增生期，24周左右发生腺瘤，32周左右出现低分化癌，55周时发现明显的前列腺癌神经内分泌分化现象。免疫表型：随着周龄的增加，TRAMP小鼠前列腺上皮细胞Tag的表达增强且增殖明显。结论TRAMP小鼠可稳定自发前列腺癌，出现前列腺上皮内瘤、高分化腺癌、低分化癌和神经内分泌癌的病理学特点，可作为人类前列腺癌研究的经典动物模型。Purpose To investigate the pathogenesis of prostate carcinoma of TRAMP mice in different time. Methods TRAMP mice were raised in SPF environment, and their survival time was recorded everyday. By using histological sections, HE staining and immu- nohistochemical staining were done to observe the pathological changes, and the expression of SV40 T antigen （Tag） and proliferating cell nuclear antigen （PCNA）. Results Generally, the survival time of TRAMP mice was 30 to 55 weeks. Proliferation could be seen in the 16th week, prostate tumor appeared in 24th week, and poorly-differentiated adenocarcinoma was found from 32nd week, while in the 55th week neuroendocrine differentiation of the prostate tumor was observed. Immunohistochemically, the glandular epiththelia with increased Tag＇s expression were obviously proliferative. Conclusions The TRAMP mouse could autogenously develop prostate carci- noma, characterized by the transition from adenoma, well-differentiated tumor, poorly-differentiated cancer to neuroendocrine adenocar- cinoma. So TRAMP mouse can be a typical animal model for prostate cancer research.

关 键 词：前列腺肿瘤 TRAMP小鼠 病理进程 

分 类 号：R364.3[医药卫生—病理学] R737.25[医药卫生—基础医学]