Danggui-shaoyao-san, a traditional Chinese medicine prescription, alleviates the orthodontic pain and inhibits neuronal and microglia activation  

作　　者：Li Hongshi Gu Zexu Wu Li'an Xia Liang Zhou Kecheng E Lingling Wang Dongsheng Kou Junping Liu Hongchen 

机构地区：[1]Institute of Stomatology, Chinese People's Libecation Army GeneralHospital, Beijing 100853, China [2]Department of Orthodontic Dentistry ,School of Stomatology, Fourth Military Medical University, Xi'an,Shaanxi 710032, China [3]Department ofPaediatric Dentistry ,School of Stomatology, Fourth Military Medical University, Xi'an,Shaanxi 710032, China [4]Department of Psychology ,School of Stomatoiogy, Fourth Military Medical -University, Xi'an,Shaanxi 710032, China [5]Department of Chinese Compound Prescription ChinaPharmaceutical University, Nanjing, Jiangsu 210038, China [6]State Key Laboratory of Natural Medicines , ChinaPharmaceutical University, Nanjing, Jiangsu 210038, China

出　　处：《Chinese Medical Journal》2014年第20期3630-3637,共8页中华医学杂志（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China （No. 30772450, No. 81170929 and No. 30873322）, the Open Project Program of State Key Laboratory of Natural Medicines, China Pharmaceutical University （No. SKLNMKF201214）. Conflict of interest： none.

摘　　要：Background The pain caused by orthodontic treatment has been considered as tough problems in orthodontic practice. Danggui-shaoyao-san （DSS） is a traditional Chinese medicine （TCM） prescription which has long been used for pain treatment and possesses antioxidative, cognitive enhancing and antidepressant effects. We raise the hypothesis that DSS exerts analgesic effect for orthodontic pain via inhibiting the activations of neuron and microglia. Methods DSS was given twice a day from day 5 prior to experimental tooth movement （ETM）. Directed face grooming and vacuous chewing movements （VCM） were evaluated. Immunofiuorescent histochemistry and Western blot analysis were used to quantify the Iba-1 （microglia activation） and Fos （neuronal activation） expression levels in the trigeminal spinal nucleus caudalis （Vc）. Results ETM significantly increased directed face grooming and VCM which reached the peak at post-operative day （POD） 1 and gradually decreased to the baseline at POD 7. However, a drastic peak increase of Fos expression in Vc was observed at 4 hours and gradually decreased to baseline at POD 7; while the increased Iba-1 level reached the peak at POD 1 and gradually decreased to baseline at POD 7. Furthermore, pre-treatment with DSS significantly attenuated the ETM induced directed face grooming and VCM as well as the Fos and Iba-1 levels at POD 1. Conclusion Treatment with DSS had significant analgesic effects on ETM-induced pain, which was accompanied with inhibition of both neuronal and microglial activation.Background The pain caused by orthodontic treatment has been considered as tough problems in orthodontic practice. Danggui-shaoyao-san （DSS） is a traditional Chinese medicine （TCM） prescription which has long been used for pain treatment and possesses antioxidative, cognitive enhancing and antidepressant effects. We raise the hypothesis that DSS exerts analgesic effect for orthodontic pain via inhibiting the activations of neuron and microglia. Methods DSS was given twice a day from day 5 prior to experimental tooth movement （ETM）. Directed face grooming and vacuous chewing movements （VCM） were evaluated. Immunofiuorescent histochemistry and Western blot analysis were used to quantify the Iba-1 （microglia activation） and Fos （neuronal activation） expression levels in the trigeminal spinal nucleus caudalis （Vc）. Results ETM significantly increased directed face grooming and VCM which reached the peak at post-operative day （POD） 1 and gradually decreased to the baseline at POD 7. However, a drastic peak increase of Fos expression in Vc was observed at 4 hours and gradually decreased to baseline at POD 7; while the increased Iba-1 level reached the peak at POD 1 and gradually decreased to baseline at POD 7. Furthermore, pre-treatment with DSS significantly attenuated the ETM induced directed face grooming and VCM as well as the Fos and Iba-1 levels at POD 1. Conclusion Treatment with DSS had significant analgesic effects on ETM-induced pain, which was accompanied with inhibition of both neuronal and microglial activation.

关 键 词：experimental tooth movement danggui-shaoyao-san FOS MICROGLIA trigeminal spinal nucleus caudalis 

分 类 号：Q813.11[生物学—生物工程] S823[农业科学—畜牧学]