大型海藻内共生真菌次生代谢产物中抑制水华蓝藻物质的分离与活性  

Identification of Compounds with Allelopathy on Bloom Microalgae from the Secondary Metabolites of Endophytic Fungi Isolated from Marine Macroalgae and the Algicidal Activities

作　　者：左进城[1] 苗凤萍[2] 王爱云[1] 胡德昌[1] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025 [2]中国科学院烟台海岸带研究所,山东烟台264025

出　　处：《植物科学学报》2014年第5期509-516,共8页Plant Science Journal

基　　金：国家"十二五"水专项(2012ZX07101007-005);国家青年科学基金项目(41106137);山东省自然科学基金(ZR2013CQ026);鲁东大学博士基金

摘　　要：为获得能明显抑制水华蓝藻的化感物质,本研究从28株大型海藻内共生真菌中筛选出了4株菌株,其粗提物能显著抑制水华鱼腥藻(Anabaena flos-aquae)和铜绿微囊藻(Microcystis aeruginosa)的生长。对这4株真菌扩大培养后,检测了从其次生代谢产物中分离出的32种单体化合物的抑藻活性。其中,化合物sterigmatocystin、(2’E,4’E,6’E)-6-(1’-carboxyocta-2’,4’,6’-triene)-11,12-epoxy-9,11-dihydroxydrim-7-ene、6,8-diO-methylnidurufin和aversin对水华鱼腥藻的抑制率显著大于50%(P<0.05),24 h半数有效浓度(EC50,24h)分别为6.865、4.302、1.824、2.319μg/mL;化合物sterigmatocystin、indol-3-carboxaldehyde和(2’E,4’E,6’E)-6-(1’-carboxyocta-2’,4’,6’-triene)-11,12-epoxy-9,11-dihydroxydrim-7-ene对铜绿微囊藻的抑制率显著大于50%(P<0.05),EC50,24h分别为4.167、5.374、8.386μg/mL。To obtain compounds with allelopathy on bloom microalgae,28 strains of marine macroalgae-endophytic fungi were tested,and four strains in which crude extracts of the secondary metabolites showed apparent allelopathy on Anabaena flos-aquae and Microcystis aeruginosa were obtained. The four strains were cultured in 15 L of PDB culture and the algicidal activities of 32 compounds isolated from the secondary metabolites were examined.The inhibition ratios of sterigmatocystin,（ 2＇E,4＇E,6＇E）-6-（ 1＇-carboxyocta-2＇,4＇,6＇-triene）-11,12-epoxy-9,11-dihydroxydrim-7-ene,6,8-di-O-methylnidurufin and aversin on A. flos-aquae were all significantly higher than 50%（ P〈0. 05）,and the EC50,24 hvalues were 6. 865 μg /mL,4. 302 μg /mL,1. 824 μg /mL and 2. 319 μg /mL,respectively. The inhibition ratios of sterigmatocystin,（ 2＇E,4＇E,6＇E）-6-（ 1＇-carboxyocta-2＇,4＇,6＇-triene）-11,12-epoxy-9,11-dihydroxydrim-7-ene and indol-3-carboxaldehyde on M. aeruginosa were all significantly higher than 50%（ P〈0. 05）,and the EC50,24 hvalues were 4.167 μg /mL,8.386 μg /mL and 5. 374 μg /mL,respectively.

关 键 词：内共生真菌 抑藻物质 水华鱼腥藻 铜绿微囊藻 化感作用 

分 类 号：X17[环境科学与工程—环境科学]