原子吸收光谱法测定血清中锂含量  

Determination of lithium in human serum by atomic absorption spectrometry

作　　者：卢永翎[1] 杭太俊[2] 李丙英[3] 王敏[3] 张义华[3] 

机构地区：[1]南京师范大学金陵女子学院,江苏南京210097 [2]中国药科大学药物分析教研室,江苏南京210009 [3]江苏恩华药业股份有限公司,江苏徐州221007

出　　处：《食品与机械》2014年第5期97-99,共3页Food and Machinery

摘　　要：建立原子吸收光谱法测定血清中锂的含量。20例健康成年男性受试者单剂量口服250mg碳酸锂片,0-72h间隔采集血样,原子吸收光谱法（AAS）测定血清中锂离子浓度,DAS 2.0计算药动学参数。结果表明,建立的AAS法在0.1-12μg/mL范围内线性关系良好,最低定量限为0.1μg/mL,批内及批间精密度RSD均小于15%。测得受试者单剂量口服250mg碳酸锂片后血清锂离子主要药动学参数如下：Cmax（2.09±0.46）μg/mL;Tmax（1.5±0.8）h;T1/2（21.8±4.9）h;AUC0-72（23.3±4.4）h·μg/mL;AUC0-＂（28.1±5.0）h·μg/mL;CL（29.1±4.9）mL/min;Vd（0.8±0.2）L/kg。该测定法操作简单,结果准确可靠,适用于锂离子的药动学研究。TO establish an AAS method for determination of lithium in human serum. Methods： 20 healthy Chinese male volunteers were given a single oral dose of 250 mg lithium carbonate and the blood samples were collected at predetermined time intervals up to 72 hours. The serum lithium concentration was determined by a valida- ted atomic absorption spectroscopic method （AAS）. The pharmaco- kinetic parameters were estimated by DAS 2. 0 software. Results： The established AAS method had linear calibration range over 0.1 12 μg/mL with a LOQ of 0.1 μg/mL for quantification of lithium in human serum. The intra- and inter batch standard deviation was less than 15%. The serum lithium pharmacokinetic parameters were as follows： Cmax（2. 09±0. 46） μg/mL; Tmax（1. 54±0. 8） h; Tw2 （21.8 ±4.9）h; AUC0-72 （23. 3±4. 4） h. μg/mL; AUC0 -∞ （28.1±5.0） h. μg/m;L CL （29. 1±4. 9） mL/min and Va（0. 8± 0.2） L/kg. Conclusions： The established AAS method is proved to be accurate and sensitive for the serum lithium determination and suitable for the lithium pharmacokinetics study.

关 键 词：碳酸锂 原子吸收光谱法 药代动力学 

分 类 号：R96[医药卫生—药理学] O657.31[医药卫生—药学]