鱼藤素降低小鼠神经母细胞瘤细胞Neuro-2A存活率的实验研究  

Inhibitory Effects of Deguelin on the Viability of Neuro-2A Cells

作　　者：熊中奎[1,2] 王思本[1] 郎娟[3] 

机构地区：[1]绍兴第二医院放疗科,浙江绍兴312000 [2]绍兴文理学院医学院临床医学部 [3]绍兴市人民医院医学研究中心

出　　处：《中国药师》2014年第11期1793-1796,共4页China Pharmacist

基　　金：浙江省自然科学基金(编号:Y2100035);浙江省中医药科技计划(编号:2011ZA108;2012ZB162);浙江省医药卫生科技计划(编号:2014KYB286)

摘　　要：目的：研究鱼藤素对小鼠神经母细胞瘤细胞Neuro-2A（N2A）存活率及凋亡的影响。方法：N2A细胞以5×10^4·ml^-1密度接种到细胞培养板中,1×10^-8mol·L^-1鱼藤素处理后6,12,24,48,72 h等5个时间点上测定细胞存活率;加入鱼藤素0,1×10^-11,1×10^-10,1×10^-9,1×10^-8,1×1^0-7,1×10^-6mol·L^-1等7个不同浓度干预48 h后测定细胞存活率。2×10^-8mol·L^-1鱼藤素干预24 h,测定细胞匀浆中caspase 3活力。结果：5×10^4·ml^-1N2A细胞以含10%胎牛血清DMEM培养基培养,第48 h达到细胞生长曲线的峰值。1×10^-8mol·L^-1鱼藤素干预24-72 h,时间依赖性显著降低N2A细胞存活率（P〈0.05）;在鱼藤素干预48 h时间点上,1×10^-8-1×10^-6·mol·^L-1浓度范围内剂量依赖性可显著降低N2A细胞存活率（P〈0.05）,其IC50=1.6×10^-8mol·L^-1。鱼藤素作用于N2A细胞24 h可显著提高caspase 3活力,约达到对照组的5.6倍。结论：鱼藤素可时间依赖性且剂量依赖性降低N2A细胞存活率,可能与增加caspase 3活力有关。Objective：To investigate the effects of deguelin on the viability and apoptosis of Neuro-2A （ N2A） cells. Methods：The cell viability of N2A cells with the density of 5 × 10^4 ·ml^-1 on the time points of 6, 12, 24, 48 and 72 hours after 1 × 10^ -8 mol ·L^-1 deguelin treatment using CCK-8 kits was determined, and that on the time point of 48 hours after 0, 1 × 10^ -11 , 1 × 10^ -10 , 1 × 10 ^-9 , 1 × 10 ^-8 , 1 × 10^ -7 , 1 × 10 ^-6 or 5 × 10^ -4 mol·L^-1 deguelin treatment was also detected. The activity of caspase 3 was determined in N2A cells treated by 2 × 10^ -8 mol·L^-1 deguelin for 24 hours. Results：N2A cells with the density of 5 × 10^4 ·ml^-1 reached the peak level in the growth curve 48 hours after the incubation in DMEM medium with 10% fetal bovine serum. The cell viability of N2A cells was decreased after the treatment of 1 × 10 ^-8 mol·L^-1 deguelin for 24-72 hours in a time-dependant manner or after the treatment of 1 × 10^ -8 -1 × 10^ -6 mol·L^-1 deguelin for 48 hours in a dose-dependant manner （P〈0. 05） with IC50 of 1. 6 × 10 ^-8 mol ·L^-1 . The activity of caspase 3 was increased after the treatment of 2 × 10^ -8 mol·L^-1 deguelin for 24 hours, which was 5. 6-fold of that in the control group. Conclusion： Cell viability of N2A cells is inhibited by deguelin in a time- and dose-dependent manner, which may be related to the activity increase of caspase 3.

关 键 词：鱼藤素 Neuro-2A细胞 细胞存活率 CASPASE 3 

分 类 号：R285.5[医药卫生—中药学]