SCD和PPAR_γ基因在延边黄牛血液中的表达及其与IMF及TG含量的关系  

Expressions of the SCD and PPAR_γ genes in blood and their relations to the IMF and TG contents in Yanbian yellow cattle

作　　者：于伽[1] 严昌国[1] 王慧明[1] 崔莲花[1] 

机构地区：[1]吉林延边大学农学院动物科学系,吉林延吉133002

出　　处：《黑龙江畜牧兽医》2014年第11期79-81,85,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：教育部博士点新教师类项目(20122201120002)

摘　　要：为了研究脂肪代谢相关基因表达在不同肉质性状延边黄牛血液中的差异,试验选择20头延边黄牛按照高档牛肉生产模式统一饲养管理,根据它们的大理石花纹等级高低分为H组和L组。应用RT-PCR方法测定延边黄牛血液中硬脂酰辅酶A去饱和酶(SCD)和过氧化氢酶体激活增殖受体γ(PPARγ)基因的表达丰度,另外分析了背最长肌肌内脂肪(IMF)和三酰甘油(TG)含量。结果表明:H组IMF和TG含量极显著和显著高于L组(P<0.01和P<0.05),H组个体血液中SCD和PPARγ表达丰度呈现出高于L组的趋势。IMF和TG含量随着大理石花纹等级的增加呈现上升趋势,SCD和PPARγ基因均可在血液中表达,并且延边黄牛血液中SCD和PPARγ基因表达水平随背最长肌IMF和TG含量的升高呈现增加的趋势。To study the differences in the expressions of the fat metabolism - related genes in the blood of Yanbian yellow cattle with different meat quality traits, twenty Yanbian yellow were selected for unified breeding and management according to the high - grade beef production mode , and divided into two groups ： the H and L groups according to the high and low levels of marbling, respectively. The method of RT - PCR was Used to determine the expression abundances of the stearoyl -eoenzyme A desaturase （SCD） and peroxisome proliferator- activated receptor - γ（ PPARγ） genes in the blood of Yanbian yellow cattle, and the contents of intramuscular fat （IMF） and triglyceride （TG） in the longissimus muscle were also analyzed. The results showed that the contents of IMF and TG in the H group were highly significantly higher and significantly higher （ P 〈 0.01 and P 〈 0.05 ） than those in the L group, the expression abundances of the SCD and PPARγ in the blood of the individuals in the H group showed a higher trend than those in the L group. The contents of IMF and TG showed a rising trend with the increase of marbling grade, and the SCD and PPARγ genes could be expressed in the blood, and the SCD and PPARγ gene expression levels in the blood of Yanbian yellow cattle showed an increasing trend with the increase of the IMG and TG contents in the longissimus muscle.

关 键 词：硬脂酰辅酶A去饱和酶(SCD)基因 过氧化氢酶体激活增殖受体γ(PPARγ)基因 肌内脂肪(IMF)含量 三酰甘油(TG) 延边黄牛 

分 类 号：S823.81[农业科学—畜牧学] Q786[农业科学—畜牧兽医]