牛巴贝斯虫lytB基因的克隆与序列分析  

Cloning and sequence analysis of the lytB gene in Babesia bovis

作　　者：刘翠翠[1] 王婧[1] 张继瑜[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所/农业部兽药创制重点实验室/甘肃省新兽药重点实验室,兰州730050

出　　处：《黑龙江畜牧兽医》2014年第11期131-133,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家现代农业肉牛牦牛产业技术体系建设专项(CARS-38);中央级科研院所基本科研业务费项目(1610322011005)

摘　　要：为了对牛巴贝斯虫lytB基因进行克隆与序列分析,试验以兰州株牛巴贝斯虫lyt B基因组为模板进行PCR扩增,将扩增得到的特异性产物克隆到p GEM-T Easy载体上,并对其进行PCR检测、酶切鉴定及序列测定分析。结果表明:试验克隆得到的864 bp基因片段与Gen Bank收录的牛巴贝斯虫lyt B核苷酸序列同源性为97.8%。说明试验成功克隆出牛巴贝斯虫lyt B基因,与Gen Bank收录的牛巴贝斯虫lyt B核苷酸序列具有高度的同源性。To perform the cloning and sequence analysis of the lytB gene in Babesia boris, the genome of the Lanzhou strains of Babesia boris was used as a template for PCR amplification. The specific amplified product was cloned into the pGEM -T Easy vector, and then the recombinant plasmid was used for PCR detection, restriction enzyme digestion and sequence analysis. The results showed that a 864 bp gene fragment was obtained by cloning, which shared high nucleotide sequence homology （97.8%） with that of the lytB gene in Babesia boris included in Gen- Bank. The results indicate that the lytB gene is successfully cloned, and shares highly homology with the nucleotide sequence of the lytB gene in Babesia bovis included in GenBank.

关 键 词：牛巴贝斯虫 lytB基因 克隆 序列分析 PCR检测 酶切鉴定 

分 类 号：S852.723[农业科学—基础兽医学] Q785[农业科学—兽医学]