超声灌注成像评价卵巢肿瘤血管生成的初步研究  

Preliminary Study of Contrast-enhanced Ultrasonography in the Evaluation of Angiogenesis in Ovarian Tumors

作　　者：杨帆[1] 杨太珠[1] 罗红[1] 田甜[1] 王静欣[1] 陈大鹏[2] 

机构地区：[1]四川大学华西第二医院超声科,成都610041 [2]四川大学华西第二医院新生儿科,成都610041

出　　处：《四川大学学报（医学版）》2014年第6期964-969,共6页Journal of Sichuan University(Medical Sciences)

基　　金：四川省科技厅科技支撑项目(No.2009SZ0181)资助

摘　　要：目的探讨超声造影灌注参数评价卵巢肿瘤血管生成的价值。方法 42例卵巢肿瘤患者术前进行超声造影灌注成像检查,术后采用免疫组化LSAB法检测卵巢肿瘤中血管内皮生长因子(VEGF)的表达情况;并采用CD34单克隆抗体标记血管内皮细胞,计算肿瘤微血管密度计数(microvessel density,MVD)。将卵巢肿瘤的VEGF阳性指数和MVD与其超声灌注参数进行相关性分析。结果卵巢肿瘤MVD和VEGF阳性指数与超声灌注参数峰值强度(PI)、曲线下面积(AUC)和半衰期(TTH)呈正相关(MVD与PI、AUC和TTH的相关系数分别为0.569、0.623和0.585;VEGF阳性指数与PI、AUC和TTH的相关系数分别为0.516、0.640和0.591)。其他灌注参数与MVD和VEGF阳性指数无相关性;高MVD与低MVD两组间PI、AUC和TTH差异有统计学意义(P均<0.05)。结论卵巢肿瘤超声灌注参数PI、AUC和TTH与肿瘤MVD和VEGF阳性指数呈正相关,能反映卵巢肿瘤的血管生成状态,为卵巢肿瘤的诊断和治疗提供了重要信息。Objective To investigate the role of contrast-enhanced ultrasonography（CEUS）with pulsed inversion harmonic imaging in the evaluation of angiogenesis in ovarian tumors.Methods Forty-two patients of ovarian tumor received CEUS examination,in which the rise time（RT）,peak intensity（PI）,area under the curve（AUC）,time from peak to one half（TTH）and time to peak（TTP）of ovarian tumors were measured.The postsurgical specimens of ovarian tumors were analysed by the stain with polyclonal antibodies against vascular endothelial growth factor（VEGF）and CD34.Correlations between the index of VEGF,microvessel density（MVD）and ultrasonic perfusion parameters were studied.Results MVD and VEGF index of ovarian tumors were significantly correlated with CEUS perfusion parameters including PI,AUCand TTH.The correlation coefficients of MVD with PI,AUCand TTH were 0.569,0.623 and 0.585respectively;and the correlation coefficients of VEGF with PI,AUCand TTH were 0.516,0.640 and 0.591 respectively.There were significant differences of the perfusion parameters between high and low MVD group.Conclusion The perfusion parameters PI,AUCand TTH in CEUS were correlated with MVD and VEGF in ovarian tumors,which may be useful in the evaluation of tumors angiogenesis.

关 键 词：卵巢肿瘤 超声造影 灌注成像 血管生成 灌注参数 

分 类 号：R737.31[医药卫生—肿瘤]