饱和氢气水对大鼠角膜新生血管抑制作用的实验研究  

The preliminary study on the inhibiting effect of saturated hydrogen water on suture induced corneal neovascularization in rats

作　　者：游玉霞[1,2,3] 梁庆丰[1,2,3] 李玉洁[1,2,3] 马科[1,2,3] 

机构地区：[1]首都医科大学附属北京同仁医院 [2]北京同仁眼科中心 [3]北京市眼科研究所眼科学与视觉科学北京市重点实验室国家眼科创新药物临床评估中心,100005

出　　处：《眼科》2014年第5期335-338,共4页Ophthalmology in China

基　　金：十二五"重大新药创制"科技重大专项基金资助项目(2011ZX09302-007-05)

摘　　要：目的探索饱和氢气水对大鼠缝线诱导角膜新生血管的抑制作用。设计实验研究。研究对象SPF级SD大鼠16只。方法 SPF级SD雄性大鼠,均选取左眼作为实验眼。成功建立缝线诱导角膜新生血管模型12只,随机分为用药组和阴性对照组。用药组给予饱和氢气水滴眼,对照组给予生理盐水滴眼,均为每次1滴,每日3次。术后第1﹑3﹑5﹑7﹑9﹑11和14天采用裂隙灯显微镜数字图像处理系统拍摄大鼠眼前节照片,通过公式计算角膜新生血管生长面积。第14天深麻醉下取大鼠眼球,HE染色观察角膜新生血管情况,并检测4-羟基壬烯酸(4-HNE)表达。主要指标角膜新生血管面积。结果第5﹑7﹑9﹑11﹑14天用药组和对照组角膜新生血管面积分别为(6.43±5.20)mm2和(11.56±4.80)mm2;(10.10±8.79)mm2和(21.39±8.57)mm2;(15.73±12.93)mm2和(33.76±13.96)mm2;(9.85±9.04)mm2和(24.80±13.06)mm2;(8.42±5.46)mm2和(23.13±13.37)mm2。除第5天两组间差异没有统计学意义(t=-1.774,P=0.106),第7﹑9﹑11﹑14天两组间差异均存在统计学意义(t=-2.252,P=0.048;t=-2.320,P=0.043;t=-2.305,P=0.044;t=-2.494,P=0.032)。结论饱和氢气水滴眼对大鼠缝线诱导角膜新生血管具有抑制作用。Objective To investigate the inhibiting effect of saturated hydrogen water on suture induced corneal neovascularization in rats. Design Experimental study. Participants Healthy adult male SD rats. Methods The left eyes of SD male rats were selected as the experimental eyes. The suture induced corneal neovascularization model was successfully induced in 12 rats, and then divided into 2 groups （n=6）. The rats of group A were given saturated hydrogen water by dropping three times a day, and those in group B were given saline by dropping three times a day for consecutive two weeks. The cornea were photographed with slit lamp photography and the area of corneal neovascularization were calculated on post-operation-day 1, 3, 5, 7, 9, 11 and 14. On post-operation-day 14, the rats were sacrificed, and then HE staining and the expression of 4-hydroxynonenal （4-HNE） protein were determined. Main Outcome Measures The area of corneal neovascularization. Results The area of corneal neovascularization of group A and B on post-operationday 5, 7, 9 , 11 and 14 were 6.43±5.20 and 11.56±4.80 mm^2, 10.10±8.79 and 21.39±8.57 mm^2, 15.73±12.93 and 33.76±13.96 mm^2, 9.85±9.04 and 24.80±13.06 mm^2, and 8.42±5.46 and 23.13±13.37 mm^2 respectively. The difference at day 5 was not significant（t=-1.774, P=-0.106）, but a significant difference was found between two groups at day 7 （t=-2.252, P=-0.048）, day 9 （t=-2.320, P=0.043）, day 11 （t=-2.305, P=-0.044）, and day 14 （t=-2.494,P=-0.032）. Conclusion Saturated hydrogen eye drops inhibit angiogenesis on suture induced corneal neovascularization.

关 键 词：饱和氢气水 角膜新生血管 抗氧化 

分 类 号：R772.2[医药卫生—眼科]