HER-2在恶性黑色素瘤中的蛋白表达、基因扩增及其临床意义  

The expression of HER-2 protein,gene amplification and its clinical significance in malignant melanoma

作　　者：李福生[1] 肖明明[2] 徐绍年[1] 杜振广[1] 王亮[1] 黄海[1] 景士兵[2] 

机构地区：[1]辽宁省人民医院骨肿瘤科,辽宁沈阳110016 [2]辽宁省人民医院病理科

出　　处：《中国美容整形外科杂志》2014年第11期700-702,共3页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的：检测恶性黑色素瘤中HER2的蛋白表达、基因扩增情况,并探讨其临床意义。方法以石蜡包埋组织芯片为材料,分别采用免疫组织化学PV9000二步法与荧光原位杂交技术检测HER2蛋白在恶性黑色素瘤、色素痣中的表达及基因扩增。结果HER2蛋白在恶性黑色素瘤、色素痣中的表达阳性率分别为76.9%、25.0%,扩增阳性率分别为75.0%、30.0%；两者比较,差异具有统计学意义（χ2=12.480,P=0）；在恶性黑色素瘤中HER2蛋白表达、基因扩增阳性率与浸润深度及淋巴结转移有关（P〈0.05）。结论HER2与恶性黑色素瘤的发生、发展有关,可以将其作为判断恶性黑色素瘤生物学行为的重要参考指标。Objective To investigate the expression of HER-2 protein and gene amplification in malignant mel-anoma and evaluation of HER-2 in malignant melanoma occurrence,development and prognosis.Methods Using PV-9000 two steps method and in situ chemical hybridization to detect the expression of HER-2 protein,gene amplification in paraffin-embedded tissue chip material.Results The positive rate of HER-2 expression in malig-nant melanoma,pigmented nevus were 76.9%,25.0%;The positive rate of HER-2 gene amplification were 75. 0%,30.0%.There were significant differences between malignant melanoma and pigmented nevus in the expres-sion and amplification of HER-2 （χ2 =12.480,P=0）;The expression and amplification of HER-2 in malignant melanoma had correlation with the depth of invasion and the lymph node metastasis （P〈0.05）.Conclusion HER-2 is closely related to the occurrence and development of malignant melanoma,and can be considered as an im-portant index for determing the biological behavior of malignant melanoma.

关 键 词：恶性黑色素瘤 HER2 免疫组织化学 荧光 

分 类 号：R739.5[医药卫生—肿瘤]