可诱导心肌特异性表达IL-22转基因小鼠模型的建立  

Establishment of myocardium-specific IL-22 expression transgenic mouse model

作　　者：程鹏飞[1] 唐景峰[2] 周立[2,3] 

机构地区：[1]武汉大学医院检验科,430072 [2]武汉大学生命科学院病毒学国家重点实验室,430072 [3]武汉大学动物实验中心/ABSL-3实验室,430072

出　　处：《免疫学杂志》2014年第11期1003-1006,1010,共5页Immunological Journal

基　　金：“中国博士后科学基金”第53批面上资助项目(2013M531745);国家自然科学基金青年项目(81301428)

摘　　要：目的建立心肌特异性、高效表达白介素22的转基因小鼠模型。方法将TRE-Tight-IL-22转基因载体显微注射入C57BL/6小鼠受精卵细胞中,得到含有其中1段转基因载体的转基因小鼠,再将这种转基因小鼠与含有能够控制TRE-Tight下游基因在心肌特异性表达的α-MHC-rtA-hGH的转基因小鼠进行交配,获得同时含有TRE-Tight-IL-22和α-MHC-rtTA-hGH这2段基因的双阳性子代转基因小鼠。使用强力霉素(Dox)持续诱导6周龄双阳性小鼠6周,再用ELISA方法检测转基因小鼠心肌细胞中IL-22表达量。结果与野生型C57BL/6小鼠比较,经过强力霉素诱导的双阳转基因小鼠心肌细胞有高表达量的IL-22(P<0.05)。结论得到了可诱导、高效表达IL-22的转基因小鼠系,为IL-22与心力衰竭关系的深入研究和治疗提供新的研究思路。To establish an in terleukin 22（IL-22） transgenic mouse model based on Tet-on inducible expression system that expressed high levle of IL-22 in cardiomyocytes, TRE-Tight-IL-22 gene vector was microinjected into the zygote of C57BL/6 mice for establishing the transgenic mice with transgenic vectors. After mating with mice containing α-MHC-rtTA-hGH which specifically controls the expression of TRE-Tight downstream gene in cardiomyocytes, a double-positive offspring with both TRE-Tight-IL-22 and α-MHC-rtTAhGH gene were obtained. And then doxycycline was added to feed the double positive transgenic mice aged over 6weeks for 6 weeks, where the concentration of IL-22 in cardiomyocytes was detected by ELISA. The transgenic mice feeding with doxycycline showed high expression levels of IL-22 in cardiomyocytes compared with wild type mice. In conclusion, this study establishes a transgenic mouse model with inducible and high level expression of IL-22, which provides a new pattern of thinking in the research and therapy routes of cardiac hypertrophy.

关 键 词：白介素22 α-肌球蛋白重链 强力霉素 Tet-on/TRE-Tight诱导表达系统 

分 类 号：R-332[医药卫生] R541.6