Zn2+对当归种子萌发和幼苗渗透调节物质积累与活性氧代谢的影响  

Effects of Zn^(2+) on seed germination,osmotica accumulation and active oxygen metabolism in seedlings of Angelica sinensis

作　　者：叶文斌[1] 

机构地区：[1]陇南师范高等专科学校农林技术学院,甘肃成县742500

出　　处：《甘肃农业大学学报》2014年第5期112-115,122,共5页Journal of Gansu Agricultural University

基　　金：甘肃省中青年基金项目(3YSO61-A25-030);陇南师范高等专科学校重点项目(2014LSZK01001)

摘　　要：以甘肃道地中药材当归(Angelica sinensis)为研究对象,通过室内砂培方法,研究了不同浓度下(10-6、10-5、10-4、10-3 mol/L)Zn2+对当归种子萌发、幼苗生长、渗透调节物质积累和活性氧代谢的影响.结果表明:低浓度Zn2+显著促进当归种子萌发和幼苗生长,高浓度Zn2+显著抑制当归种子萌发和幼苗生长,抑制程度随Zn2+浓度提高而增强;随着Zn2+浓度的增加,叶片O2-·产生速率和H2O2含量明显增加,SOD、POD、CAT和APX活性先升后降,高浓度Zn2+处理下GSH和AsA含量明显降低,幼苗叶片中Pro、可溶性糖和MDA含量随Zn2+浓度升高而升高.Sand culture experiment was conducted to study the effects of Zn2＋ with different concentra-tions （10-6 mol/L,10-5 mol/L,10-4 mol/L,10-3 mol/L）on the germination and seedling growth,osmotica accumulation and active oxygen metabolism of Angelicasinensis,authentic Chinese herbal medicine of Gan-su.The results showed that the low concentrations of Zn2＋ increased the seeds germination and seedling growth significantly,while the high concentrations of Zn2＋ showed inhibition effects,and the inhibition de-gree increased with the concentration.With the increasing of Zn2＋ concentration,the O2 -·production rate and H2 O2 contents of leafves increased obviously,the activities of superoxide dismutase,peroxidase,cata-lase and ascorbate peroxidase increase first and then decreased.The contents of glutathione and ascorbic acid of leaves decreased obviously under high concentration of Zn2＋.The proline,soluble sugar and malon-aldehyde contents increased with the increasing of Zn2＋ concentration.

关 键 词：当归 Zn2+ 渗透调节物质 活性氧代谢 

分 类 号：Q945[生物学—植物学] S567.53[农业科学—中草药栽培]