葡萄幼苗在PEG-6000模拟干旱胁迫条件下生理生化的响应  

Response of Polythylene Glycol (PEG-6000) Simulated Water Stress on Characteristics of the Physiological Indicators in Grape

作　　者：许雯博[1] 彭玉梅[1] 王清风 于坤[1] 荣新民 郁松林[1] 

机构地区：[1]石河子大学/新疆兵团果蔬重点生理实验室,新疆石河子832003 [2]新疆石河子葡萄研究所,新疆石河子832003

出　　处：《新疆农业科学》2014年第8期1504-1511,共8页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目(30760144);新疆生产建设兵团重点攻关项目(1360464)

摘　　要：【目的】探讨葡萄幼苗在不同干旱胁迫程度条件下,随着胁迫时间的增加,其水分代谢、渗透调节物质以及根系活力的变化。【方法】采用0%、5%、10%、15%四种不同浓度的PEG-6000模拟干旱胁迫,分别在胁迫12、36、60、84和108 h测定其叶片的相对含水量、水分饱和度、自由水与束缚水比值、根系活力、电导率和脯氨酸的变化。【结果】在轻度干旱胁迫T1下,在胁迫前60 h,植株叶片的相对含水量、自由水下降缓慢,束缚水、相对电导率、脯氨酸含量呈缓慢上升趋势。但在中度干旱胁迫T2和重度干旱胁迫T3下,随着胁迫时间的增加,相对含水量、自由水等快速下降,束缚水、相对电导率、脯氨酸呈快速上升趋势,一直到达峰值,而后呈下降趋势。【结论】在干旱胁迫前期植株可以通过增强根系活力和自身的水分、渗透的调节,来抵御外界干旱胁迫。【Objective】In order to explore water metabolism,osmotic adjustment substance,root activity＇s change laws of Chardonnay and Flame seedless in different drought stresses with the increase of stress time. 【Method】The experiments were conducted with 0%,5%,10%,15% four levels of different concentrations PEG6000 simulated drought stress,and RWC,WSD,free water,bound water,root activity,proline content＇s change on the stress time 12 hours,36 hours,60 hours,84 hours and108 hours were measured.【Result】Plant leaves＇ RWC,free water declined slowly and WSD,bound water,relative conductivity,proline content slow increased slowly in the light drought stress T1 and stress time 60 hours; plant leaves＇ RWC,free water,dropped rapidly and WSD,bound water,relative conductivity,proline content increased quickly until it reached the peak and then displayed downward tendency in the middle stress drought T2 and heavy drought stress T3. 【Conclusion】Under the drought stress,early plants ＇ resistance to outside influence could be strengthened by regulating the water metabolism and adjusting the osmosis.

关 键 词：葡萄 弗雷无核 霞多丽 干旱胁迫 生理指标 

分 类 号：S663.1[农业科学—果树学]