云南野生稻抗稻瘟病Pi-ta基因的检测及分析  

Identification and Analysis on the Rice Blast Resistance Gene Pi-tain Wild Rice from Yunnan,China

作　　者：赵国珍[1] 蒋春苗[2] 刘吉新[1] 陈于敏[1] 余腾琼[2] 程在全[2] 

机构地区：[1]云南省农业科学院粮食作物研究所,昆明650205 [2]云南省农业科学院生物技术与种质资源研究所,昆明650223

出　　处：《中国水稻科学》2014年第6期675-680,共6页Chinese Journal of Rice Science

基　　金：云南省科技攻关计划资助项目(2012BB013);NSFC计划资助项目(U1302265;3160067);农业行业专项(201003021);云南省应用基础研究重点项目(2012FA005)

摘　　要：以16个不同来源的云南野生稻作为供试材料,用抗稻瘟病Pi-ta基因特异引物(KG2/KG4)进行PCR扩增,并对PCR产物进行克隆、测序及分析。结果表明,16个野生稻中7个品种持有抗稻瘟病Pi-ta基因,其中,来自云南景洪的紫秆普通野生稻中Pi-ta基因的编码区与原始型的抗稻瘟病Pi-ta基因的DNA序列完全相同。可见,云南可能是抗稻瘟病基因Pi-ta的起源地之一。A total of 1 6 accessions of wild rice from different regions of Yunnan Province were used for PCR amplifica-tion with Pi-ta gene specific primers.The amplified PCR products were cloned into Topo cloning vector,and se-quenced.Among 1 6 accessions,the identical sequence fragment to the resitance Pi-ta gene was amplified separately from 7 accessions.The Pi-ta allele in one accession of Oryza rufipogon with purple stem from Jinghong contains an i-dentical DNA sequence in the coding region to the original Pi-ta resistance gene.It suggested that Yunnan might be one of the origins of the Pi-ta gene.

关 键 词：野生稻 抗稻瘟病Pi-ta基因 克隆 测序分析 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治] S511.034[农业科学—植物保护]