糖丁酸梭状芽孢杆菌BAA-117利用不同碳源发酵产燃料丁醇的研究  

Research of biobutanol fermented by Clostridium saccharobutylicum BAA-117 using different carbon fuel

作　　者：彭牡丹[1] 王义强[1,2,3] 王启业[1] 陆利[1] 

机构地区：[1]中南林业科技大学生物技术实验室,湖南长沙410004 [2]经济林培育与保护教育部重点实验室,湖南长沙410004 [3]国家林业局生物乙醇研究中心,湖南长沙410004

出　　处：《可再生能源》2014年第11期1703-1709,共7页Renewable Energy Resources

基　　金：国家林业局"948"项目(2011-4-13)

摘　　要：利用木质纤维原料替代传统粮食原料发酵可降低丁醇生产成本。先将杨木纤维进行酶解糖化,然后利用糖丁酸梭状芽胞杆菌BAA-117进行丁醇发酵。研究结果表明:BAA-117对单糖的利用率为混合糖(葡萄糖27.5 g/L,阿拉伯糖3 g/L,木糖19.5 g/L)>葡萄糖>木糖>阿拉伯糖;杨木蒸汽爆破渣通过酶解后产生的总还原糖含量为46.8 g/L,经过72 h无添加发酵,丙酮、丁醇与乙醇总溶剂含量为6.6 g/L,其中丁醇产量为4.8 g/L,ABE总溶剂的生产速率为0.089 g/(L·h);在补充培养基后,ABE总溶剂含量为9.53 g/L,其中丁醇产量为6.88g/L,ABE总溶剂的生产速率为0.13 g/(L·h);添加20 g/L的混合糖后,丁醇产量达到7.28 g/L,ABE总溶剂的生产速率为0.141 3 g/(L·h),比单杨木纤维发酵丁醇产量提高了58.8%。Lignocellulosic material was an alternative to traditional food ingredients to reduce pro- duction costs of butanol. In this study, the enzymatic hydrolysate of poplar wood fibre was used by Clostridium saccharobutylicum BAA-11 as substrate to produce butanol. The results showed that the utilization rate of sugar by Clos tridium saccharob utylic um BAA- 11 was that ： mixed sugar （glu- cose 27.5 g/L, arabinose 3 g/L, xylose 19.5 g/L）〉 glucose〉 xylose〉 arabinose; The total reducing sugars content of poplar fibe hydrolysate was 46.8 g/L, and the total ABE content in system was 6.6 g/L, of which butanol was 4.8 g/L after 72 h fermentation, the ABE productivity was 0.089 g/（L＇h）. The y!eld of butanol and productivity of total ABE were 6.88 g/L and 0.13 g/（L-h） respectively after adding medium, and the total ABE content was 9.53 g/L; After adding 20 g/L mixed sugar, the yield of butanol was improved to 7.28 g/L, and the productivity of total ABE was 0.141 3 g/（L·h）. Consequently, the yield of butanol increased 58.8% than single poplar fiber hydrolysate fermenta- tion.

关 键 词：杨木纤维 酶解 厌氧发酵 丁醇 糖丁酸梭状芽胞杆菌 

分 类 号：TK6[动力工程及工程热物理—生物能] S216.2[农业科学—农业机械化工程]