不同剂量复方青黛颗粒对溃疡性结肠炎模型大鼠血清IL-6、IL-10水平的影响  

Effects of different dosage of compound indigo granule on serum IL-6 and IL-10 in rat model of ulcerative colitis

作　　者：刘伟 刘刚[2] 冷骏峰 

机构地区：[1]湖北省直属机关医院药剂科,湖北武汉430071 [2]湖北省人民医院药剂科,湖北武汉430060 [3]迈德高武汉生物医学信息科技有限公司,湖北武汉430075

出　　处：《实用临床医药杂志》2014年第19期4-6,共3页Journal of Clinical Medicine in Practice

基　　金：中国高校医学期刊临床专项资金(11321403)

摘　　要：目的观察不同剂量复方青黛颗粒对溃疡性结肠炎(UC)模型大鼠血清白细胞介素-6(IL-6)、白细胞介素-10(IL-10)水平的影响。方法将70只SD大鼠随机分为6组:空白对照组、模型对照组、柳氮磺胺吡啶(SASP)组、低剂量组(300 mg/kg)、中剂量组(600 mg/kg)及高剂量组(1 200 mg/kg)。采用三硝基苯磺酸(TNBS)法制备UC大鼠模型,造模成功后各实验组分别给予SASP及不同剂量复方青黛颗粒灌胃,对照组给予等量生理盐水。连续给药10 d后观察各组大鼠给药后结肠组织病理学评分及血清IL-6、IL-10水平的差异。结果空白对照组结肠组织病理学评分显著低于其他各组(P<0.01),模型对照组显著高于其他各组(P<0.05或P<0.01),SASP组与高剂量组之间均有显著差异(P<0.01),不同剂量组之间有显著差异(P<0.05或P<0.01)。方差分析表明,空白对照组血清IL-6、IL-10水平与其他各组均有显著差异(P<0.01),各给药组血清IL-6、IL-10水平均较模型对照组有不同程度改善,其中SASP组及高剂量组IL-6水平显著低于其他组(P<0.05),高剂量组IL-10水平显著高于其他各组(P<0.05)。结论复方青黛颗粒可有效改善UC模型大鼠的症状,降低IL-6的表达,并增加IL-10的表达,维持促炎性细胞因子与抗炎性细胞因子之间的平衡。Objective To observe the effects of different dosage of compound indigo granules（ CIG） on levels of serum Interleukin-6（ IL-6） and IL-10 in in rat model of ulcerative colitis（ UC）.Methods A total of 70 rats were randomly divided into six groups,including blank control group,model control group,Salazosulfapyridine（ SASP） group,low,medium and high dosage of CIG group.Rat model of ulcerative colitis were prepared by trinitro-benzene-sulfonic acid（ TNBS） and administrated with SASP and different dosage of CIG.Equivalent normal saline were given to control group.histopathological score,serum IL-6 and IL-10 were observed 10 days after successive administration.Results Histopathological score was lowest（ P〈0.01） in blank control group and highest in model group（ P〈0.05,P〈0.01）.there was a significant difference between SASP group and high dosage group（ P〈0.01）,and among different dosage groups（ P〈0.05,P〈0.01）.Variance analysis indicated the difference of serum IL-6 and IL-10 between blank control group and other groups（ P〈0.01）.the level of IL-6 in SASP group and high dosage group was significant lower than other groups（ P〈0.05）,and IL-6 in high dosage group was higher than other groups（ P〈0.05）.Conclusion CIG has potential to improve the condition in rats rat model of ulcerative colitis by inhibiting IL-6 expression,enhancing IL-10 expression,and balancing the immune inflammation.

关 键 词：溃疡性结肠炎 复方青黛颗粒 细胞因子 白细胞介素-6 白细胞介素-10 

分 类 号：R574.62[医药卫生—消化系统]