蒿甲醚对人恶性胶质瘤细胞的抑制作用  

Inhibitory effect of artemether on human malignant glioma cells

作　　者：胡宜[1] 王英滨[1,2] 薛一雪[3] 王萍[3] 刘云会[1] 

机构地区：[1]中国医科大学附属盛京医院神经外科,辽宁沈阳110001 [2]沈阳医学院奉天医院神经外科,辽宁沈阳110001 [3]中国医科大学基础医学院神经生物教研室,辽宁沈阳110001

出　　处：《实用临床医药杂志》2014年第19期48-51,共4页Journal of Clinical Medicine in Practice

基　　金：国家自然科学基金资助项目(81302191;81172197;81272564)

摘　　要：目的探讨蒿甲醚对U251人胶质瘤细胞增殖活性、凋亡的影响及其机制。方法将浓度为0、50、100、200、400、600及800μmol/L的蒿甲醚孵育U251胶质瘤细胞24、48 h。利用MTT法测定细胞增殖活性。然后将利用浓度为0、200、400及800μmol/L的蒿甲醚培养U251人胶质瘤细胞48 h,利用Annexin V-FITC/PI法检测细胞凋亡。最后利用Western blot法检测胶质瘤细胞的磷酸化AKt的表达水平。结果 400μmol/L及更高浓度的蒿甲醚培养48 h,U251细胞的增殖活性受到显著抑制,同时随着浓度升高抑制作用增强。与对照组相比,蒿甲醚显著促进U251细胞的凋亡。400μmol/L和800μmol/L浓度的蒿甲醚显著抑制了磷酸化Akt的表达水平。结论蒿甲醚能够以剂量依赖的方式显著抑制U251细胞的增殖活性并促进其凋亡,该抑制作用可能与其抑制Akt信号通路有关。Objective To explore the effects of artemether on the proliferative activity and apoptosis of human glioma cells and discuss the possible mechanism.Methods U251 glioma cells were treated with artemether at the concentrations of 0,50,100,200,400,600 and 800 μmol / L for24 or 48 hours.Cellular proliferative activity was measured with MTT assay.Artemethers of 0,200,400 and 800 μmol / L were added and the cellular apoptosis was investigated by Annexin V-FITC / PI method.Finally the expression level of phospholate Akt was checked by Western blot assay.Results Under the treatment of artemether at 400 μmol / L and cultured for 48 hours,the proliferative activity of U251 human glioma cells was inhibited significantly and the inhibitory effects were stronger with higher concentrations.The apoptosis was promoted significantly as well.Artemether of 400 μmol / L and 800μmol /L inhibited the expression of p-Akt significantly compared with the control group.Conclusion Artemether inhibits the viability of U251 human glioma cells and promotes the apoptosis in a dose-depend manner.Akt signaling pathway might be involved in this process.

关 键 词：蒿甲醚 胶质瘤 增殖 凋亡 信号通路 

分 类 号：R739.4[医药卫生—肿瘤]