人凝血酶原复合物中凝血因子Ⅸ效价检测中影响因素的探讨  

Study on the affecting factors in the analysis of coagulation factor Ⅸ activity in human prothrombin complex concentrates

作　　者：李青[1] 刘晓[1] 张璘[1] 包正琦[1] 窦姿 张安山[1] 张金[1] 何彦林[1] 

机构地区：[1]兰州生物制品研究所有限责任公司甘肃省疫苗技术研究中心,甘肃兰州730046

出　　处：《中国输血杂志》2014年第11期1249-1251,共3页Chinese Journal of Blood Transfusion

摘　　要：目的探讨人凝血酶原复合物(PCC)中凝血因子Ⅸ效价检测的影响因素,优化检测条件。方法研究不同的Ca2+浓度对一期法检测凝血因子Ⅸ效价的影响,并分别考察磷酸三丁酯、聚山梨酯-80(S/D)以及硫酸鱼精蛋白中和肝素对检测的影响。结果 Ca2+浓度为0.012 5 mol/L时,检测结果最稳定,标准曲线的相关性(r2)为0.999±0.001,回收率为(99.2±1.789)%,5次重复试验的变异系数为1.80%;S/D对PCC样品中凝血因子Ⅸ效价检测的影响无统计学意义(P>0.1);PCC样品的肝素未中和时,稀释倍数的影响较大,中和后凝血因子Ⅸ效价受稀释倍数影响较小。结论优化了凝血因子Ⅸ效价检测的影响因素,最优Ca2+离子浓度为0.012 5 mol/L;S/D试剂无影响;硫酸鱼精蛋白中和肝素后,凝血因子Ⅸ效价受稀释倍数影响较小。Objective To study the affecting factors in the analysis of coagulation factor Ⅸ activity in human prothrombin complex concentrates,and to optimize testing conditions. Methods The influence of different Ca2 ＋concentrations,triethyl phosphate,polysorbate-80（ S / D） and heparin on the analysis of coagulation factor Ⅸ activity was investigated. Results Analysis appeared to be the most reliable when Ca2 ＋concentration was 0. 012 5 mol / L. The correlation coefficient of standard curve was 0. 999 ± 0. 001. The rate of recovery was 99. 2% ± 1. 789%. The variation coefficient of five repeated tests was 1.8%. S / D reagents did not exemplify a prominent influence on the analysis of coagulation factor Ⅸ activity（ P 0. 1）. The result of analysis could be greatly influenced by dilution effects if heparin did not undergo neutralization. Conclusion The conditions for analysis were optimized. The optimum Ca2 ＋concentration was 0. 125 mol / L. S / D reagents did not carry significant influence on the analysis. Heparin should be neutralized before the analysis.

关 键 词：CA^2+浓度 S/D 肝素中和 凝血因子Ⅸ效价 

分 类 号：R457.1[医药卫生—治疗学]