2012年山东省肉鸡生产加工环节沙门菌污染水平及耐药分析  

Contaminant levels and drug resistance analysis of Salmonella isolated from broiler production and processing course in Shandong Province in 2012

作　　者：张华宁[1] 陈玉贞[1] 侯配斌[1] 王勤[2] 胡光春[3] 李心朋 王梅[1] 毕振旺[1] 

机构地区：[1]山东省传染病预防控制重点实验室山东省疾病预防控制中心,济南250014 [2]淄博市疾病预防控制中心 [3]济南市疾病预防控制中心

出　　处：《卫生研究》2014年第6期933-938,共6页Journal of Hygiene Research

基　　金：山东省医药卫生科技发展计划(No.2011HZ057,2013WS0163);国家科技重大专项子课题(No.2012ZX10004215)

摘　　要：目的调查山东省肉鸡孵化、养殖、屠宰、流通等环节沙门菌的污染程度及抗生素耐药谱的分布情况。方法 2012年在济南和淄博选择种鸡场、孵化厂、养殖场、屠宰场、大型超市和农贸市场,共采集2496份样品,按照GB 4789.4—2010进行沙门菌鉴定和血清学分型,微量肉汤稀释法检测抗生素的耐药分布情况。结果肉鸡孵化环节阳性率为2.39%,养殖环节为12.67%,屠宰环节为27.00%,配送分销环节为22.72%。分离菌株共包含32种血清型,其中印第安纳沙门菌占42.25%,肠炎沙门菌占34.21%。95.51%的分离菌株至少对一种抗生素耐药,71.37%的分离菌株对3种以上的抗生素耐药,28.21%的分离菌株对10种以上的抗生素耐药,7株对14种抗生素全部耐药,均为印第安纳沙门菌。结论济南和淄博市肉鸡生产加工各环节沙门菌污染严重,其中屠宰环节和分销配送环节沙门菌污染率高于孵化环节和养殖环节;肉鸡中沙门菌耐药形势严峻。Objective To investigate the Salmonella contamination and its antibiotic resistance spectrum in the whole process of broiler hatching, cultivation, slaughtering, processing,distribution and retail in Shandong Province. Methods 2496 samples were collected from the chicken farms, hatching factory, breeding farm, slaughterhouse, large supermarkets and farmers＇ market in Jinan and Zibo City. All samples were tested according to GB 4789.4--2010 and the antimicrobial susceptibilities by the broth microdilution method. Results The positive rate of the hatching process was 2.39% ,the breeding process was 12.67% , the slaughter process was 27.00% , the distribution and retail process was 22.72%. There were 32 serotypes, the Indiara and Enteritidis Salmorella accounted for 42.25% and 34.21% respectively. 95.91% of the Salmorella strains were resistant to at least one antibiotic, 71.37% of the strains were resistant to at least three antibiotics , 28.21% of the strains were resistant to at least ten antibiotics,7 strains were resistant to fourteen antibiotics, all of them are Indiana Salmonella. Conclusion In Jinan and Zibo City, it is seriously contaminated by various aspects of Salmonella in broiler production and processing. The contamination rate of slaughter and Salmonella distribution is higher than the links of hatching process and breeding. The situation of broiler Salmonella is becoming severe.

关 键 词：沙门菌 生产加工环节 血清学 耐药 肉鸡 

分 类 号：R155.55[医药卫生—营养与食品卫生学] TS207.5[医药卫生—公共卫生与预防医学]