鸟苷酸环化酶C在大肠癌患者外周血中的表达及其临床意义  

Expression and significance of guanylyl cyclase C in colorectal carcinoma

作　　者：戴庆福[1,2] 姜添荣[1,3] 林玉梅[1,2] 曹春芳[1,2] 

机构地区：[1]福建医科大学附属龙岩第一医院 [2]福建省龙岩市第一医院中心实验室,福建龙岩364000 [3]福建省龙岩市第一医院消化内科,福建龙岩364000

出　　处：《分子诊断与治疗杂志》2014年第6期398-401,共4页Journal of Molecular Diagnostics and Therapy

基　　金：国家科技部863计划(2011AA02A110)

摘　　要：目的探讨鸟甘酸环化酶C(GCC)在大肠癌患者外周血中的表达及其临床意义。方法采用RT-PCR法对32例大肠癌患者、25例大肠良性病变患者、30例健康体检者外周血中GCC表达情况进行检测,并对大肠癌病理指标与GCC mRNA表达阳性率之间关系进行研究。结果 32例大肠癌患者中,GCC mRNA的阳性检出率为65.63%(21/32),良性组中GCC mRNA的阳性检出率为20.00%(5/25),而对照组中未见GCC mRNA表达;三组外周血中表达阳性率差异具有统计学意义(P<0.05)。大肠癌患者GCC mRNA表达水平与Duke分期、淋巴结转移和肝转移密切相关(P<0.05),与年龄、性别、肿瘤大小等无关(P>0.05)。结论 GCC mRNA在大肠癌患者外周血中阳性表达率较高,特别是复发或转移病例,可作为大肠癌术后复发、转移的早期检测指标之一。Objective To explore the expression and its clinical significance of GCC in colorectal carcinoma, and provide a theoretical reference for the clinical prevention and treatment. Methods To study the relationship between pathological indexes and the positive expression rate of GCC mRNA in colorectal carcinoma, 32 cases of colorectal cancer, 25 patients with benign colorectal lesions, 30 cases of healthy donors were detected in GCC expression by RT-PCR method. Results The positive rate of GCC mRNA in 32 cases of colorectal cancer patients was 65.63%（21/32）, while in benign group was 20% （5/25）. However, the GCC mRNA was unexpressed in the control group. The different peripheral blood expression rates of the three groups were statistically significant （P 〈 0.05）. The GCC expression of patients with colorectal cancer is closely related to Duke stage, lymph node metastasis and liver metastasis （P 〈 0.05）, and independent of age, gender and tumor size （P〉0.05）. Conclusion The positive expression rate of GCC mRNA is high in peripheral blood of patients with colorectal cancer, especially in the recurrent or metastatic cases. And for this, it can be used as one of indexes of early detection of recurrence, metastasis after operation.

关 键 词：大肠癌 阳性表达率 临床意义 

分 类 号：R735.34[医药卫生—肿瘤]