上海地区汉族人群NADPH氧化酶p22phox编码基因CYBA—A930G多态性与高血压性脑出血的相关性  

Correlation between NADPH oxidase p22phox CYBA -A930G polymorphism and hypertensive cerebral hemorrhage in Chinese Han population in Shanghai area

作　　者：袁州[1] 赵迎春[1] 

机构地区：[1]南京医科大学附属上海松江中心医院神经内科,上海201600

出　　处：《国际脑血管病杂志》2014年第10期751-755,共5页International Journal of Cerebrovascular Diseases

基　　金：上海市松江区卫生局领先合作项目A类（2011LX03）

摘　　要：目的研究上海汉族人群中NADPH氧化酶p22phox亚基-A930G基因多态性与高血压性脑出血的相关性。方法纳入高血压性脑出血患者和正常对照者，采用聚合酶链反应和限制性片段长度多态性技术检测NADPH氧化酶p22pb“亚基-A930G基因型和等位基因。结果共纳入高血压脑出血患者128例和对照组151例。高血压脑出血组收缩压、舒张压、血糖和三酰甘油水平以及吸烟和饮酒的患者构成比显著性高于对照组（P均〈0．05），AA、AG和GG基因型（42．2％、44．5％、13．3％对63．6％、27．8％、8．6％；x212．757，P=0．002）以及A、G等位基因（64．5％、35．5％对77．5％、22．5％；X2：8．734，P=0．001）频率与对照组均存在显著性差异。多变量logistic回归分析显示，收缩压≥140mmHg（1mmHg=0．133kPa）[优势比（oddsratio，OR）13．952，95％可信区间（confidence interval,CI．242～26．879；P〈0．001]、载脂蛋白A≥0．99mmol／L（OR 3．139，95％CI 1．012～9．733；P=0．048）和AG＋GG基因型（OR2．333，95％CI 1．253～4．342；P=0．008）为高血压性脑出血的独立危险因素。结论在中国上海地区汉族人群中，NADPH氧化酶D22phox亚基～A930G多态性是高血压性脑出血的独立危险因素。Objective To investigate the correlation between the NADPH oxidase p22phox -A930G polymorphism and hypertensive intracerebral hemorrhage in Chinese Han population in Shanghai area. Methods The patients with hypertensive intracerebral hemorrhage and normal controls were enrolled. The polymerase chain reaction and the polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） were used to detect the genotypes and alleles of NADPH oxidase p22phox - A930G. Results A total of 128 patients with hypertensive intracerebral hemorrhage and 151 healthy controls were enrolled. The levels of systolic blood pressure, diastolic blood pressure, glucose, and triacylglycerol, as well as the proportions of smoking and alcohol consumption in patients of the hypertensive intracerebral hemorrhage group were significantly higher than those of the control group （all P〈 0.05）. There were significant differences in AA, AG, and GG genotypes （42.2%, 44. 5%, and 13.3% vs. 63.6%, 27. 8%, and 8.6%, X2 = 12. 757, P =0. 002） and in A and G allele （64. 5% and 35.5% vs. 77.5% and 22.5% ; X2 = 8. 734, P = 0. 001 ） frequencies between the hypertensive intracerebral hemorrhage group and the control group. Multivariate logistic regression analysis showed that systolic blood pressure ≥140 mm Hg （1mm Hg =0. 133 kPa）（odds ratio [OR] 13.952, 95% confidence interval [CI] 7.242-26.879; P〈 0.001）, apolipoprotein A ≥ 0. 99 mmol/L （OR 3. 139, 95% CI 1. 012 -9. 733; P =0. 048）, and AG ＋GG genotype （OR 2. 333, 95% CI 1. 253- 4. 342; P= 0. 008） were the independent risk factors for hypertensive intracerebral hemorrhage. Conclusions Among the Chinese Hart population in Shanghai area, the NADPH oxidase p22phox -A930G polymorphism is an independent risk factor for hypertensive intracerebral hemorrhage.

关 键 词：脑出血 高血压NADPH氧化酶 多态现象 遗传学 活性氧 危险因素 

分 类 号：R743.34[医药卫生—神经病学与精神病学]