过表达核心蛋白聚糖基因对胆管癌细胞迁移及侵袭的影响  

Effect of decorin gene overexpression on migration and invasion of cholangiocarcinoma cells

作　　者：王伟林[1] 余翔[1] 李泉[1] 毛永欢 沈佳佳[1] 骆霞岗[1] 喻春钊[1] 

机构地区：[1]南京医科大学第二附属医院普通外科,210011

出　　处：《中华实验外科杂志》2014年第11期2381-2383,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30972910、81172269）;中国博士后一等基金资助项目（20060390294）;江苏省自然科学基金资助项目（BK2011858）;江苏省六大人才高峰基金资助项目（WSW-032）

摘　　要：目的 观察过表达核心蛋白聚糖（DCN）基因对胆管癌细胞迁移及侵袭功能的影响.方法 脂质体介导真核表达质粒pEGFP-DCN和空载体pEGFP-N1转入人胆管癌细胞株QBC939,Western blot检测DCN蛋白以及E-钙黏蛋白的表达,逆转录-聚合酶链反应（RT-PCR）检测DCNmRNA以及E-钙黏蛋白mRNA的表达,Transwell实验检测细胞迁移、侵袭能力.结果 RT-PCR结果显示过表达DCN较pEGFP-N1上调32.34倍;Western blot结果显示过表达组DCN上调2倍.Transwell结果显示转染pEGFP-DCN的胆管癌细胞迁徙和侵袭能力较转染pEGFP-N1分别降低约56.9％和40.2％;转染pEGFP-DCN与pEGFP-N1胆管癌细胞中E-钙黏蛋白的表达水平,结果可见较转染pEGFP-N1体组胆管癌细胞比较,转染pEGFP-DCN组E-钙黏蛋白的表达水平明显升高.结论 DCN过表达可抑制QBC939细胞的迁移与侵袭能力,可能与升高E-钙黏蛋白表达相关.Objective To investigate the effects of decorin （DCN） gene overexpression on the migration and invasion of human cholangiocarcinoma cells.Methods The pEGFP-DCN plasmids and pEGFP-N1 control plasmids were transfected into the human cholangiocarcinoma cell line QBC939 cells.The mRNA and protein expression of decorin and E-cadherin in QBC939 cells transfected with plasmid overexpressing decorin was detected by reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting respectively.Transwell assay was used to observe the migration and invasion.Results Compared to the control plasmids,RT-PCR showed the mRNA expression of decorin in the pEGFP-DCN plasmids was increased by 32.34 fold.Western blotting showed the protein expression of decorin was increased by 2 fold.Transwell assay showed that the migration and invasion capacity of the QBC939 cells transfected with pEGFP-DCN was decreased by 56.9% and 40.2% respectively as compared with the control cells.The expression level of E-cadherin was significantly increased after DCN transfection into QBC939 cells as compared with the control cells.Conclusion After the over-expression of DCN gene,the migration and invasion capacity of the QBC939 cells was effectively suppressed,which may be associated with vthe elevated E-cadherin expression.

关 键 词：胆管癌 核心蛋白聚糖 迁移 侵袭 

分 类 号：R735.8[医药卫生—肿瘤]