双氢青蒿素抑制人胆管癌生长的研究  

The in vivo study of dihydroartemisinin inhibits the growth of human cholangiocarcinoma

作　　者：陈有挺[1] 洪志鹏[1] 周智[1] 欧荣文[1] 石铮[1] 

机构地区：[1]福建医科大学附属第一医院肝胆胰外科福建省腹部外科研究所,福州350005

出　　处：《中华实验外科杂志》2014年第11期2386-2388,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 双氢青蒿素治疗人胆管癌QBC939裸鼠种植瘤的作用并探讨其可能的作用机制.方法 建立人胆管癌QBC939裸鼠种植瘤动物模型,随机分3组：双氢青蒿素组（DHA）、空白对照组（生理盐水）、溶剂对照组（植物油）,对比分析各组裸瘤重量、抑瘤率、缺氧诱导因子（HIF）-1α的表达、计数微血管密度（MVD）和凋亡指数（AI）.结果 DHA组的种植瘤体积（1 979.63±357.29） mm^3]和重量[（1.77±0.23）g]明显小于对照组[种植瘤体积（2 827.69±549.00） mm^3,重量（2.50±0.65）g,P＜0.05];实验组的HIF-1α的表达强度（0.2280±0.0439）少于对照组（0.336 7±0.089 6,P＜0.05）;实验组的MVD值（11.06±1.44）低于对照组（17.44±2.24,P＜0.05）;实验组肿瘤组织的凋亡指数[AI,（13.18±2.42）％]明显大于对照组[（3.28±1.04）％,P＜0.05].结论 双氢青蒿素对人胆管癌裸鼠种植瘤生长具有抑制作用,其作用机制可能与诱导肿瘤细胞凋亡和抑制肿瘤血管生成有关.Objective To investigate the effect and the potential mechanisms of dihydroartemisinin on the treatment of human cholangiocarcinoma cell line QBC939 xenograft in nude mouse.Methods A total of 18 animal models of nude mouse xenograft by human cholangiocarcinoma cell line QBC939 were established and randomly divided into three groups：the dihydroartemisinin group （DHA）,the control group （saline）,and the solvent control group （vegetable oil）.The weight of tumor,the tumor inhibition rate,the expression of hypoxia-inducible factor （HIF）-1α,the microvessel density （MVD） and the apoptotic index （AI） in each group were compared and analyzed.Results The volume and the weight of xenograft tumor in DHA group [（1 979.63 ± 357.29） mm^3 and （1.77 ± 0.23） g] were significantly lower than those in the control group [（2 827.69 ± 549.00） mm^3 and （2.50 ± 0.65） g,P 〈 0.05].The expression of HIF-1α in the experimental group （0.228 0 ± 0.043 9）was less than that in the control group （0.336 7 ± 0.089 6,P 〈 0.05）.MVD in the experimental group （11.06 ± 1.44） was less than that in the control group （17.44 ± 2.24,P 〈 0.05）.The AI in the experimental group [（13.18 ± 2.42） %] was significantly greater than that in the control group [（3.28 ± 1.04） %,P 〈 0.05].Conclusion Dihydroartemisinin has an inhibitory effect on the tumor growth of human cholangiocarcinoma in nude mice,and the mechanism may be related to the induction of the apoptosis of tumor cells,and the inhibition of tumor angiogenesis.

关 键 词：胆管癌 双氢青蒿素 缺氧诱导因子-1Α 体内实验 

分 类 号：R735.8[医药卫生—肿瘤]