音猬因子信号通路在小鼠急性胰腺炎中的作用  

Role of Sonic Hedgehog signal pathway in mice acute pancreatitis

作　　者：杨辉[1] 施森[1] 郑英强[1] 陈霞[2] 周翔宇[1] 

机构地区：[1]泸州医学院附属医院血管外科,四川646000 [2]泸州医学院附属医院消化内科,四川646000

出　　处：《中华实验外科杂志》2014年第11期2434-2436,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81100272）;泸州市科技局科研基金资助项目

摘　　要：目的 观察音猬因子（Shh）信号通路在小鼠急性胰腺炎中的作用.方法 雨蛙素腹腔注射诱导急性胰腺炎模型后,引入Hedgehog信号的拮抗剂环杷明（Cyclopamine）,用逆转录-聚合酶链反应（RT-PCR）、Western blot方法检测Shh在胰腺组织的表达,同时检测了胰腺髓过氧化物酶（MPO）水平.结果 与对照组比较,雨蛙素注射1h后,Shh表达逐渐升高,在24h到达最高.与对照组比较[（231.13 ±10.03） U/mg],MPO活性逐渐升高,6h到达高峰[（1 255.56±126.68） U/mg],24h恢复正常[（230.48±17.69） U/mg].当Cyelopamine抑制Hedgehog信号后,胰腺炎症明显加重.结论 在急性胰腺炎性过程中,Shh起重要的保护作用.Objective To study the potential role of Sonic Hedgehog （Shh） signal pathway in mice acute pancreatitis.Methods Acute pancreatitis was induced by intraperitoneal injections of cerulean 7 times at hourly intervals in mice,and the specific antagonist of Hedgehog was injected as well.Shh expression was detected by real-time reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry （IHC）.The level of pancreatic myeloperoxidase （MPO） was determined.Results As compared with control group,RT-PCR and Western blotting results showed Shh in experimental group was increased,at 24 h,up to peak values.Inflammation was assessed by the activity of MPO.In control group,the MPO activity was （231.13 ± 10.03） U/mg,and experimental group showed a rapid increase in MPO activity,at 6 h,up to peak values [（1 255.56 ± 126.68） U/mg].The MPO activity recovered baseline at 24 h [（230.48 ± 17.69） U/mg].When hedgehog signal was inhibited by cyclopamine,the inflammation of pancreas was more serious.Conclusion During the acute pancreatitis,Shh signal may play a protective role to regulate the pancreatic inflammation.

关 键 词：急性胰腺炎 音猬因子信号通路 环杷明 

分 类 号：R576[医药卫生—消化系统]