甲状腺乳头状癌组织与癌旁组织中长链非编码RNA表达谱分析  

Study on expression profile of long non-coding RNA in thyroid papillary carcinoma

作　　者：杜培准 李宇[1] 周岩冰[1] 吕亮[2] 谭斌[1] 孙腾[1] 焦学龙[1] 周金哲[1] 

机构地区：[1]青岛大学附属医院普外科,266003 [2]北京大学人民医院

出　　处：《中华实验外科杂志》2014年第11期2548-2550,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81270449）;山东省自然科学基金资助项目（ZR2012HM046）

摘　　要：目的 建立甲状腺乳头状癌组织与癌旁组织中长链非编码RNA （lncRNA）差异表达谱.方法 分别提取5例甲状腺乳头状癌患者癌组织和癌旁组织中的RNA,体外逆转录制备并标记双链DNA,用含有39000条lncRNA的双通道基因芯片检测,对原始数据进行归一化处理及差异分析.结果 芯片筛选出差异表达的lncRNA 26 924条.有明显差异的lncRNA共245条（变化＞2倍,P ＜0.05）,这其中在肿瘤组织中高表达的IncRNA 73条,低表达的lncRNA 172条.变化在3倍以上的lncRNA共40条,差异在4倍以上的lncRNA有2条.结论 甲状腺乳头状癌组织与癌旁组织比较,其lncRNA表达谱发生明显改变,提示lncRNA可能参与甲状腺乳头状癌的发生.Objective To analysis the expression profile variation of long non-cording RNA （lncRNA） in thyroid papillary carcinoma and normal thyroid tissue.Methods Total RNA from the thyroid papillary carcinoma tissue and normal thyroid tissue of 5 patients with thyroid papillary were prepared respectively.Hybridization was performed with the profile chip containing 39 000 human lncRNAs.After the scanning of Agilent scanner,data were normalized and analyzed by using the Agilent GeneSpring software.Results Compared with normal thyroid tissue,26 923 lncRNAs expressed differentially,245 lncRNAs were significantly differential （fold change 〉 2 ; P 〈 0.5） in thyroid papillary carcinoma tissue,in which 73 lncRNAs were up-regulation and 172 lncRNAs were down-regulation.Conclusion Obvious changes of lncRNA expression profile were observed in the pathogenesis of thyroid papillary carcinoma,lncRNA may be related to the progress of thyroid papillary carcinoma.

关 键 词：甲状腺乳头状癌 长链非编码RNA 芯片 

分 类 号：R736.1[医药卫生—肿瘤]