鄂西北周边汉族人群CODIS数据库基因座等位基因的分布频率调查和分析  

Alleles Distribution Survey and Analysis of Gene Locus in CODIS Database of Han Population in Northwest of Hubei Province

作　　者：王晓勋[1] 李瑞明[2] 陈敏[3] 贺娇[4] 尹霞[4] 胡巧林 梅俊[4] 吴惠超 

机构地区：[1]湖北医药学院附属太和医院检验部 [2]湖北医药学院附属太和医院生物医学研究所 [3]十堰市西苑医院 [4]湖北医药学院医疗系,湖北十堰442000

出　　处：《湖北医药学院学报》2014年第5期464-468,共5页Journal of Hubei University of Medicine

摘　　要：目的:本实验收集鄂西北周边汉族人群做亲权鉴定人的标本,确认无血缘关系的个体作为该地区随机抽样人群,检测其CODIS数据库中所有13个等位基因座中各等位基因的分布频率。方法:应用Chelex提取DNA,Amp FISTR Identifiler试剂盒扩增,毛细管电泳分型。对每个等位基因座等位基因分布进行统计计算,检测每个等位基因座哈德温伯格平衡(Hard-Wenborger平衡)。结果:在被检测的387位无关个体中13个CODIS等位基因座共检出148个等位基因型以及13个等位基因座中等位基因的分布频率。结论:为本地区等位基因数据库的建立提供第一手的等位基因频率和相关统计学资料。应用适合本地人群的等位基因频率可以提高亲权鉴定和个体识别中累积父权指数的可靠性。Objective To collect the samples of Han poplulation around northwest of Hubei province for paternity testing,the unrelated individuals were selected as a random sample of a northwest population in Hubei province,then to analyze the allele frequency of 13 allelic loci in CODIS database. Methods The Chelex-extracting DNA method,Amp FISTR Identifilerbased method for gene amplification,and DNA typing by capillary electrophoresis were used to detect these samples,respectively. The allele frequency were calculated in every allelic loci of these samples,and the Hard-Weinberg equilibrium of them were also analyzed. Results The 148 allelotype and 13 distribution frequencies of allelic loci were detected from 13 CODIS allelic loci of 387 unrelated individuals. Conclusion This research has established the valuable first-hand database on the allele frequency and related statistical data in the region,which could provide beneficial help for improving the reliability of paternity identification and cumulative paternity index in the local population.

关 键 词：CODIS 等位基因座 等位基因频率 哈德温伯格平衡 

分 类 号：R440[医药卫生—诊断学]