梗阻性黄疸患者血清对人PASMCs增殖、迁移及表型转换的影响  

Effects of serum in patients with obstructive jaundice on proliferation, migration and phenotype modulation of human pulmonary arterial smooth muscle cells

作　　者：杨勇[1] 陈兵[1] 陈杨[1] 祖宝利[1] 易斌[1] 鲁开智[1] 

机构地区：[1]第三军医大学第一附属医院麻醉科,重庆市400038

出　　处：《中华麻醉学杂志》2014年第10期1271-1274,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81170053,81170414）

摘　　要：目的 探讨梗阻性黄疸患者血清对人肺动脉平滑肌细胞（PASMCs）迁移、增殖及表型转换的影响.方法 分离人PASMCs,并传代培养,将培养的人PASMCs分别用梗阻性黄疸患者血清和健康志愿者血清孵育,于孵育24、48和72 h时采用CCK-8法和细胞划痕实验分别检测细胞增殖能力和迁移能力,采用Western blot法检测平滑肌-α-肌动蛋白（SM-α-actin）和钙调蛋白表达水平.结果 与健康志愿者血清孵育的人PASMCs比较,经梗阻性黄疸患者血清孵育的PASMCs增殖能力和迁移能力增强,孵育24h时钙调蛋白表达上调,孵育48、72 h时SM-α-actin和钙调蛋白表达下调（P＜0.05）;与孵育24 h时比较,经梗阻性黄疸患者血清孵育48 h时人PASMCs增殖能力和迁移能力增强,SM-α-actin表达上调,钙调蛋白表达下调（P＜0.05）;与孵育48 h时比较,经梗阻性黄疸患者血清孵育72 h时人PASMCs迁移能力增强（P＜0.05）,增殖能力、SM-α-actin和钙调蛋白表达差异无统计学意义（P＞0.05）.结论 梗阻性黄疸患者血清可增强人PASMCs增殖和迁移能力,并促进其向合成表型的转换.Objective To evaluate the effects of the serum in the patients with obstructive jaundice on the proliferation,migration and phenotype modulation of human pulmonary arterial smooth muscle cells （PASMCs）.Methods PASMCs were isolated from the patients,underwent passage and were then subcultured.The cultured PASMCs were incubated with the serum in the patients with obstructive jaundice or with the serum in the healthy volunteers.At 24,48 and 72 h of incubation,the proliferation and migration of the cells were determined by CCK-8 assay and wound healing assay,respectively,and Western blot analysis was used for detection of smooth muscleα-actin （SM-α-actin） and calponin protein expression in human PASMCs.Results The proliferation and migration of human PASMCs were significantly enhanced,calponin protein expression was up-regulated at 24 h of incubation,and the SM-α-actin and calponin protein expression was down-regulated at 48 and 72 h of incubation when human PASMCs were incubated with the serum in the patients with obstructive jaundice as compared with those when the cells were incubated with the serum in the healthy volunteers.When human PASMCs were incubated with the serum in the patients with obstructive jaundice,the proliferation and migration of the cells were significantly enhanced,SM-α-actin expression was up-regulated and calponin protein expression was down-regulated at 48 h of incubation as compared with those at 24 h of incubation.When human PASMCs were incubated with the serum in the patients with obstructive jaundice,the migration of the cells was significantly enhanced,and no significant change was found in the proliferation and SM-α-actin and calponin protein expression at 72 h of incubation as compared with those at 48 h of incubation.Conclusion The serum in the patients with obstructive jaundice can enhance the proliferation and migration of human PASMCs and promotes synthetic PASMC phenotype.

关 键 词：黄疸 阻塞性 肌细胞 平滑肌 肺 细胞增殖 细胞运动 细胞分化 

分 类 号：R575[医药卫生—消化系统]