高效液相色谱法测定花生和玉米中的环匹阿尼酸  

Determination of cyclopiazonic acid in peanut and corn by high performance liquid chromatography

作　　者：诸葛庆[1] 葛乐勇[1] 李博斌[1] 范洋波[1] 曾红燕[1] 黄晓丽[1] 夏炜芳[1] 

机构地区：[1]绍兴市质量技术监督检测院,国家黄酒产品质量监督检验中心,绍兴312071

出　　处：《食品科技》2014年第11期335-339,共5页Food Science and Technology

基　　金：浙江省质量技术监督系统科研计划项目(20120205)

摘　　要：建立一种高效液相色谱法测定花生和玉米中环匹阿尼酸含量的方法。样品经液液萃取净化处理,采用反相C18色谱柱Gemini C18（4.6 mm×250 mm,5μm）,以乙腈：4%乙酸水溶液（三氟乙酸调pH值2.2）=55：45为流动相,流速1 mL/min,检测波长280 nm,对花生和玉米中的环匹阿尼酸进行检测。在50、100、200μg/kg添加量范围内,花生和玉米的平均回收率分别为83.9%～98.5%和86.3%～102.3%,方法的检出限为8μg/kg,线性范围为0.3～12.0μg/mL（R＾2=0.9998）。所建立的方法快速、简便、准确,可以作为花生和玉米中环匹阿尼酸的检测方法。This study developed a reverse phase HPLC method for determing cyclopiazonic acid in peanut and corn. After samples were pretreated by liquid-liquid extraction, cyclopiazonic acid was separated on a Gemini C18（4.6 mm×250 mm, 5 μm） using a mobile phase composed of acetonitrile and 4% acetic acid（pH 2.2 adjusted with trifluoacetic acid）（55：45, v/v） at a flow rate of 1 mL/min and detected using a UVdetector set at 280 nm. At the spike levels of 50, 100, 200 μg/kg, the average recoveries for cyclopiazonic acid from peanut were between 83.9% and 98.5%, the average recoveries for cyclopiazonic acid from corn were between 86.3% and 102.3%. The limit of detection was 8 μg/kg for both peanut and corn, and the linear range was from 0.3~12 μg/mL（R^2=0.9998）. The method was fast, easy and accurate. Therefore, the method developed can be used to determine cyclopiazonic acid in peanut and corn.

关 键 词：高效液相色谱法 环匹阿尼酸 花生 玉米 

分 类 号：O657.72[理学—分析化学]