三倍体枇杷茎尖培养与植株再生  

Plant Regeneration from Shoot Tips in Triploid Loquat

作　　者：陶炼[1] 潘翠萍[1,2] 谢红江[1] 王永清[2] 杨文渊[1] 杨芩[3] 李靖[1,2] 陈栋[1] 涂美艳[1] 刘伟[1] 江国良[1] 

机构地区：[1]四川省农业科学院园艺研究所,四川成都610066 [2]四川农业大学园艺学院,四川雅安625014 [3]凯里学院环境与生命科学学院,贵州凯里556000

出　　处：《热带作物学报》2014年第11期2249-2254,共6页Chinese Journal of Tropical Crops

基　　金：省财政基因工程专项资金项目:果树生物技术研究与创新(No.2011JYGC05-018);国家公益性行业(农业)科研专项经费项目:枇杷产业技术研究与试验示范(No.201003073)

摘　　要：以三倍体枇杷茎尖为外植体,研究了取材时间及茎尖大小、植物生长调节剂对三倍体枇杷茎尖培养与植株再生过程中萌发、伸长、生根及移栽的影响。结果表明:春季取材且茎尖大小为0.7 cm时,茎尖成活率最高,为77.5%;初代培养适宜培养基为MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+IBA 1.5 mg/L,在此培养基上,三倍体枇杷茎尖萌发率高达91%;茎尖伸长适宜培养基为MS+6-BA 0.5 mg/L+NAA 0.3 mg/L+GA30.3 mg/L,平均芽苗高度可达4.9 cm;芽苗增殖适宜培养基为MS+6-BA 1.0 mg/L+NAA 0.3 mg/L+Tryptone 750 mg/L,增殖系数为7.7;生根适宜培养基为1/2MS+NAA 0.25 mg/L+IBA 2.0 mg/L,生根率为73.3%;三倍体枇杷组培苗移栽入腐熟有机肥∶园土∶锯末(1∶2∶1)的基质中成活率达到93.67%。Plant regeneration was completed for the first time using shoot tips as the explants harvested from a triploid mother plant in loquat. A series of experiments were carried out to investigate the effects of sampling time and the size of the explants, growth regulators on shoot tip sprouting, elongation, rooting and transplantation. The results showed that sampling in spring and shoot-tip size of 0.7 cm were appropriate, resulting in a survival rate of 77.5%. Appropriate initial culture medium was MS＋6-BA 1.0 mg/L＋NAA 0.5 mg/L＋NAA 1.5 mg/L, in which the sprouting rate reached 91%. Suitable medium for shoot elongation was MS＋6-BA 0.5 mg/L＋NAA 0.3 mg/L＋GA3 0.3 mg/L, which resulted in an average shoot height of 4.9 cm. The most suitable medium for shoot proliferation was MS＋6-BA 1.0 mg/L＋NAA 0.3 mg/L＋Tryptone 750 mg/L, in which a high multiplication coefficient of 7.7 was obtained. The most suitable medium for rooting was 1/2MS＋NAA 0.25 mg/L＋IBA 2.0 mg/L, in which the rooting rate was 73.3%. The rooted plantlets, after hardening, survived at a rate of 93.67% in the transplantation matrix of decomposed organic fertilizers, garden soil and sawdust （1：2：1） under careful temperature and water management.

关 键 词：三倍体枇杷 茎尖培养 植株再生 

分 类 号：S667.304[农业科学—果树学]