山蒌单芽茎段培养与快繁技术研究  

Single-node Stem Culture and Rapid Propagation of Piper sarmentosum

作　　者：李新[1,2] 黄东益[1,2] 成善汉[3] 唐燕琼[1,2] 刘进平[1,2] 

机构地区：[1]海南大学农学院 [2]海南大学热带作物种质资源保护与开发利用教育部重点实验室,海南海口570228 [3]海南大学园艺园林学院,海南海口570228

出　　处：《热带作物学报》2014年第11期2255-2259,共5页Chinese Journal of Tropical Crops

基　　金：海南大学地方服务项目“海南特色蔬菜新品种选育与推广利用”;国家自然科学基金项目“海南野生胡椒种质资源遗传多样性分析和保护利用”(No.31060204)

摘　　要：以山蒌单芽茎段为外植体,对其进行组织培养和快速繁殖研究。结果表明:山蒌嫩茎最佳消毒方法为70%酒精消毒30 s,再用0.1%升汞溶液消毒9 min;适合腋芽萌发的启动培养基为MS+0.5 mg/L NAA+3 mg/L 6-BA;继代增殖培养最适宜的培养基为MS+0.1 mg/L NAA+3 mg/L 6-BA;试管苗在1/2MS+3 mg/L NAA+0.1-0.5 mg/L6-BA生根较好;山蒌试管苗移栽成活率可以达到95%以上。Tissue cuhure and rapid propagation of Piper sarmentosum were conducted with single-node stems as the explants. The results showed that the best surface sterilization was achieved by immersing the explants in the solution of 70% alcohol for 30 seconds then in 0.1% HgCl2 solution for 9 minutes. The medium （MS＋0.5 mg/L NAA＋3 mg/L 6-BA） was suitable for starting culture of P. sarmentosum. Successful shoot proliferation was obtained on MS supplemented with 0.1 mg/L NAA and 3 mg/L 6-BA. The optimal rooting medium was 1/2MS＋3 mg/L NAA＋0.1-0.5 mg/L 6-BA.

关 键 词：山蒌 组织培养 快速繁殖 表面消毒 

分 类 号：TS971[轻工技术与工程]