氯氰菊酯对小鼠肾组织的氧化损伤  

Oxidative damage induced by cypermethrin in the kidney tissue of Kunming mice

作　　者：马萍[1,2] 张忠杰[1] 焦铭[1] 廖文莉[1] 陈姣娥[1] 杨旭[2] 武阳[1,2] 

机构地区：[1]湖北科技学院基础医学院,湖北咸宁437100 [2]华中师范大学生命科学学院环境生物医学实验室,湖北武汉430079

出　　处：《中国环境科学》2014年第11期2958-2963,共6页China Environmental Science

基　　金：湖北省教育厅科学技术研究项目(D20122803);教育部国家级大学生创新训练计划项目(201210927036)

摘　　要：以昆明小鼠为受试动物,随机分为6组,包括1个阴性对照组、3个氯氰菊酯染毒组、1个维生素E组和1个高剂量氯氰菊酯加维生素E组,染毒组按10,20,40mg/kg水平,维生素E的剂量为100mg/kg,灌胃染毒小鼠7d.以肾组织匀浆测定活性氧(ROS)、还原型谷胱甘肽(GSH)、丙二醛(MDA)和8-羟基脱氧鸟苷(8-OHd G)的含量;以肾组织细胞测定DNA-蛋白质交联(DPC)系数.随着氯氰菊酯染毒剂量的升高,肾组织的ROS、MDA、8-OHd G含量和DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系.染毒剂量为20mg/kg时,MDA和8-OHd G含量差异有统计学意义(P<0.05);染毒剂量为40mg/kg时,ROS(F=3.7044)、GSH(F=3.4908)、MDA(F=3.5851)、8-OHd G含量(F=11.7934)和DPC系数(F=6.9165)差异均有统计学意义(P<0.05,P<0.01).病理学观察可见20,40mg/kg剂量组小鼠肾小球增生肥大,肾小管上皮细胞水肿,管腔变小.与高剂量染毒组相比较,高剂量染毒加维生素E组肾组织的ROS、MDA含量、8-OHd G含量和DPC系数均有下降,GSH含量上升(P<0.05,P<0.01).高剂量(≥20mg/kg)的氯氰菊酯能造成小鼠肾组织的氧化损伤,维生素E有抗氧化作用.Kunming mice were randomly grouped into six groups and treated with orally administered drugsona daily base for a week. The groups included one solvent control group, three cypermethrin groups, one high dose cypermethrin plus vitamin E protection group and one vitamin E group. The exposure doses of cypermethrin groups were 10, 20 and 40mg/kg respectively. Some kidney tissues were then made into homogenates for the measurement of reactive oxygen species（ROS）, glutathione（GSH）, 8-hydroxy-2-deoxyguanosine（8-OHd G） and malondialdehyde（MDA） contents. Meanwhile, DNA-protein crosslink（DPC） coefficients were detected from brain cell suspension.The kidney contents of ROS, MDA, 8-OHd G and DPC coefficients increased gradually in a dose-dependent manner, whereas GSH content decreased accordingly. In the exposure group with the dose of 20mg/kg, MDA contents and DPC coefficients were significantly higher than those of the control group（P〈0.05）; ROS（F=3.7044）, GSH（F=3.4908）, MDA（F=3.5851）, 8-Ohd G（F=11.7934） and DPC（F=6.9165） levels were significantly different in levels of each biomarker between 40mg/kg group and control group（P〈0.05,P〈0.01）. Furthermore, the high-dosaged（20and 40mg/kg） group had increased glomerulus cells, proliferation and hypertrophy of glomerulus, hydrops of renal tubular epithelial cell, and narrow lumen. Administration of vitamin E（VE） to cypermethrin-treated mice reflected that ROS, MDA, 8-OHd G and DPC increased whereas GSH decreased（P〈0.05,P〈0.01）. Cyermethrin at certain doses（≥20mg/kg） could induce oxidative stress in mice kidney, whereas vitamin E had antioxidant effects.

关 键 词：氯氰菊酯 维生素E 活性氧 还原型谷胱甘肽 丙二醛 8-羟基脱氧鸟苷 DNA-蛋白质交联 氧化损伤 

分 类 号：X171.5[环境科学与工程—环境科学]